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餐厨垃圾与秸秆混合厌氧消化可有效改善两者单独厌氧消化易出现的挥发性脂肪酸积累和木质纤维素难以降解等问题,并回收生物质能.在中温(35℃)和高温(55℃)条件下,对餐厨垃圾与秸秆混合厌氧消化进行了序批式试验研究.结果表明,进料的挥发性固体(VS)浓度为3 kg·m~(-3),中温条件下,物料进料比(VS/VS)为9∶1时,单位有机负荷累积甲烷产量达到最高,为272.0 mL·g~(-1);高温条件下,进料比为5∶5时,单位有机负荷累积甲烷产量达到最高,为402.3 mL·g~(-1),分别显著高于两温度条件下餐厨垃圾单独厌氧消化的结果(中温218.6 mL·g~(-1),高温322.0 mL·g~(-1)).高温条件下物料中的碳流向甲烷的比例高于中温,且两物料混合消化促进碳流向甲烷.高温下木质纤维素总降解率为34.7%~45.8%,高于中温的12.6%~42.2%.利用高通量测序技术检测细菌与古菌的16S rRNA基因序列信息和真菌的内转录间隔(ITS)序列信息,结果表明,高温下木质纤维素降解细菌和放线菌数量明显高于中温条件,可解释高温下木质纤维素总降解率更高的原因.
The combined anaerobic digestion of food waste and straw can effectively improve the accumulation of volatile fatty acids and lignocelluloses which are easily anaerobically digested by both anaerobic digestion and biomass recovery, and recover biomass energy at medium temperature (35 ℃) and high temperature (55 ℃) ℃) under the conditions of anaerobic digestion of food waste and straw mixed batch test results show that the feed of volatile solids (VS) concentration of 3 kg · m ~ (-3), the temperature conditions , When the ratio of feedstock to feedstock (VS / VS) was 9:1, the cumulative methane production per unit organic load was the highest, which was 272.0 mL · g -1. Under high temperature conditions, when the feed ratio was 5: 5, unit The cumulative organic methane production reached the highest level of 402.3 mL · g -1, which was significantly higher than that of single anaerobic digestion (218.6 mL · g -1) and 322.0 mL · g -1), the ratio of carbon to methane in the material under high temperature was higher than that in medium temperature, and the two materials mixed and digested to promote carbon flow to methane.The total degradation rate of lignocellulose was 34.7% -45.8% at high temperature, Higher than the 12.6% ~ 42.2% of the moderate temperature.Using high-throughput sequencing technology to detect 16S rRNA gene sequence of bacteria and archaea and fungal internal transcribed spacer (ITS) Column information, results showed that, at high temperatures lignocellulolytic bacteria and actinomycetes significantly higher than the temperature conditions due to higher total degradation of lignocellulosic interpretable at a high temperature.