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目的探讨肝癌p33ING1bmRNA表达水平及与临床病理特征的关系,揭示p33ING1b基因在肝癌发生发展中的作用及临床诊断意义。方法用逆转录聚合酶链反应(RT-PCR)检测55例原发性肝癌患者癌组织及其癌旁组织p33ING1bmRNA水平。结果63·6%(35/55)癌组织及70·9%(39/55)癌旁组织p33ING1bmRNA呈阳性表达,癌组织p33ING1bmRNA表达量为0·410±0·175,明显低于癌旁组织0·529±0·203,两者比较差异有统计学意义(P<0·05)。p33ING1bmRNA水平与肝癌的病理分级、淋巴结转移、包膜浸润与否有关,而与年龄、性别、肿瘤大小、癌栓形成、合并肝硬化与否无关。结论p33ING1b表达下调在肝癌发生、发展中起重要作用,可以作为肝癌分化、侵袭性及预后的评价指标。
Objective To investigate the relationship between the expression of p33ING1b mRNA and clinicopathological features in hepatocellular carcinoma (HCC), and to reveal the role of p33ING1b in the occurrence and development of hepatocellular carcinoma (HCC) and its clinical significance. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect p33ING1b mRNA in 55 cases of primary liver cancer and its adjacent tissues. Results The positive expression of p33ING1b mRNA was found in 63.6% (35/55) of cancerous tissues and 70.9% (39/55) of paracancerous tissues, and the expression of p33ING1b mRNA in cancerous tissues was significantly lower than that in paracancerous tissues 0 · 529 ± 0 · 203, the difference was statistically significant (P <0 · 05). The level of p33ING1b mRNA was related to the pathological grade of liver cancer, lymph node metastasis and infiltration of the capsule, but not with age, sex, tumor size, tumor thrombus formation and cirrhosis. Conclusion The down-regulation of p33ING1b plays an important role in the occurrence and development of hepatocellular carcinoma and can be used as an index to evaluate the differentiation, invasion and prognosis of hepatocellular carcinoma.