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为明确噻虫胺对桃蚜Myzus persicae(Sulzer)的毒力和桃蚜的代谢解毒机制,本研究采用点滴法、叶片浸渍法和叶柄内吸法分别测定了噻虫胺对桃蚜的毒力,以及胡椒基丁醚(PBO)、磷酸三苯酯(TPP)和顺丁烯二酸二乙酯(DEM)对噻虫胺毒力的影响;检测了噻虫胺在亚致死剂量LC6,LC15和LC30下对桃蚜体内乙酰胆碱酯酶、羧酸酯酶和谷胱甘肽-S-转移酶活力的影响。结果表明:噻虫胺对桃蚜点滴、浸渍和内吸LC50分别为1.891,2.341和1.303mg/L;3种酶抑制剂分别与噻虫胺按1∶1混用,PBO对噻虫胺增效达2.41倍,增效作用显著;TPP对噻虫胺增效达1.52倍,增效作用也较明显;DEM对噻虫胺无增效作用。以噻虫胺LC30浓度处理桃蚜,处理后24h其体内乙酰胆碱酯酶比活力受到显著抑制,抑制率达41.2%;以LC15和LC30浓度的噻虫胺处理桃蚜,处理后24h其体内羧酸酯酶比活力分别是对照的1.29和1.36倍,有显著诱导激活作用;以噻虫胺LC6,LC15和LC30浓度处理的桃蚜,对其体内谷胱甘肽-S-转移酶的抑制率分别达7.9%,11.9%和22.7%。结果说明噻虫胺对桃蚜具有较高毒力,羧酸酯酶和多功能氧化酶可能是桃蚜体内代谢噻虫胺的主要酶系。
In order to clarify the virulence of clothianidin against Myzus persicae (Sulzer) and the metabolic detoxification of Myzus persicae, the virulence of clothianidin to Myzus persicae was determined by spotting method, leaf dipping method and petiole suction method , And the effects of piperonyl butoxide (PBO), triphenyl phosphate (TPP) and diethyl maleate (DEM) on the toxicity of clothianidin. The effects of clothianidin on the cytotoxicity of sublethal LC6, LC15 and Effect of LC30 on the Activities of Acetylcholinesterase, Carboxylesterase and Glutathione-S-Transferase in Peach Aphids. The results showed that the relative concentrations of clothianid aphid (1.481, 2.341 and 1.303mg / L) were applied to Myzus persicae respectively. The three kinds of enzyme inhibitors were mixed with clothianidin at a ratio of 1: Up to 2.41 times, synergistic effect significantly; TPP synergistic to clothianidin up to 1.52 times, the synergistic effect is more obvious; DEM did not synergistic to clothianidin. The myzus persicae was treated with clothianidin LC30 for 24 hours. The specific activity of acetylcholinesterase was inhibited by 41.2% after treated with clothianidin LC30 for 30 days. After treated with clothianidin at LC15 and LC30 for 24 hours, Esterase specific activity of 1.29 and 1.36 times the control, respectively, a significant induction of activation; with clothianidin LC6, LC15 and LC30 concentrations of Myzus persicae in vivo inhibition of glutathione-S-transferase were Up 7.9%, 11.9% and 22.7%. The results showed that clothianidin had higher virulence to Myzus persicae, and carboxylesterase and multifunctional oxidase might be the major enzymes in the metabolism of clothianidin in Myzus persicae.