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目的建立高效液相色谱(HPLC)方法同时测定中药金银花中咖啡酸及木犀草苷的含量。方法采用Agilent C18色谱柱(250mm×4.6mm,5.0μm),流动相采用甲醇:0.1%的磷酸水溶液,按照梯度洗脱方式,流速为1.0ml/min,进样量为15μl,检测波长为338nm,柱温为25℃。结果咖啡酸与木犀草苷分别在8.1-82.0μg/ml、2.1-42.0μg/ml范围内线性关系良好,线性回归方程分别为Y=37.71X-265.77(R=0.9997),Y=15.56X+3.98(R=0.9995)。结论采用HPLC方法测定金银花中的有效成分咖啡酸、木犀草苷,方法简单、分离效果好,重复性好,是一种较好的检测金银花药材质量的方式。
Objective To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of caffeic acid and luteolin in Chinese honeysuckle. Methods The Agilent C18 column (250 mm × 4.6 mm, 5.0 μm) was used. The mobile phase consisted of methanol: 0.1% phosphoric acid in water. The gradient elution was performed at a flow rate of 1.0 ml / min. The injection volume was 15 μl and the detection wavelength was 338 nm The column temperature was 25 ℃. Results There was a good linear relationship between caffeic acid and luteolin in the range of 8.1-82.0μg / ml and 2.1-42.0μg / ml. The linear regression equations were Y = 37.71X-265.77 (R = 0.9997), Y = 15.56X + 3.98 (R = 0.9995). Conclusion HPLC method for the determination of the active ingredient in honeysuckle Luteolin, luteolin, simple, good separation, good reproducibility, is a good way to detect honeysuckle medicinal quality.