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目的通过比较肝门部胆管癌与胆总管中下段癌的全基因组表达差异,进一步揭示胆管癌发生、发展的内在分子机制。方法采用含21329条Oligo DNA的人类全基因组寡核苷酸芯片,对3例肝门部胆管癌与正常胆管配对检测差异表达基因,另取4例胆总管中下段癌标本与正常胆管配对检测差异表达基因,通过SAM分析,得到两者间差异表达基因,并采用实时定量RT-PCR验证芯片结果。结果肝门部胆管癌与胆总管中下段癌之间基因表达既存在共性,又存在差异。与正常胆管组织相比,两者共同上调基因244条,共同下调基因399条;而两者差异表达基因共82条(ratio≥2.0或≤0.5)。通过SAM分析(q=0),两者显著差异表达基因共40条,其中上调基因29条,下调基因11条,包括AREG、EPHA2、SPP1、PACE4等。结论高通量的基因芯片技术能够筛选出大量的胆管癌差异表达基因,肝门部胆管癌与胆总管中下段癌的基因表达亦存在着明显的聚类性质差别。
Objective To compare the whole genome expression between hilar cholangiocarcinoma and common bile duct carcinoma to further reveal the intrinsic molecular mechanism of cholangiocarcinoma. Methods Using 21329 Oligo DNA human genome-wide oligonucleotide microarray, 3 cases of hilar cholangiocarcinoma and normal bile duct paired detection of differentially expressed genes, and the other 4 cases of common bile duct cancer specimens and normal bile duct paired detection difference The genes were expressed and the differentially expressed genes were obtained by SAM analysis. The real-time quantitative RT-PCR was used to verify the chip results. Results The gene expression between hilar cholangiocarcinoma and common bile duct carcinoma was not only common but also different. Compared with normal bile duct tissue, both of them up-regulated 244 genes and down-regulated 399 genes in common, while 82 differentially expressed genes (ratio≥2.0 or ≤0.5). By SAM analysis (q = 0), a total of 40 genes were significantly differentially expressed, including 29 up-regulated genes and 11 down-regulated genes including AREG, EPHA2, SPP1 and PACE4. Conclusion High-throughput gene chip technology can screen a large number of differentially expressed genes in cholangiocarcinoma. There is also a clear clustering difference in the gene expression between hilar cholangiocarcinoma and common bile duct carcinoma.