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目的 :探讨宽叶缬草 ( VOL)对蛛网膜下腔出血 ( SAH)后脑血管痉挛的作用 ,并与尼莫通 ( Nim)进行对照。方法 :采用枕大池二次注血法复制兔 SAH模型 ,用 VOL 5 0 0 mg· kg-1及Nim6mg· kg-1每 8h 1次 ,口服给药 ,治疗 5 d后用组织化学方法分别显示兔 SAH基底动脉及海马CA1区微血管 ,用 CMIAS图像分析系统及体视学方法对基底动脉管径和微血管密度进行定量分析 ,并观察海马 CA1区组织病理学变化 ,评价 VOL对 SAH后脑血管痉挛的作用。结果 :SAH后 5 d基底动脉管径明显缩小 ,海马 CA1区微血管长度密度、体积密度明显下降 ,海马 CA1区神经元变性坏死 ,VOL治疗后基底动脉管径、微血管密度恢复正常 ,神经元少有坏死 ,与 SAH组比较有显著性差异 ,其作用大小与 Nim相比差异不显著。结论 :VOL能扩张脑基底动脉 ,改善微循环 ,保护神经元 ,是值得进一步研究开发的中草药
Objective: To investigate the effect of VOL on the cerebral vasospasm after subarachnoid hemorrhage (SAH) and compare it with Nim. METHODS: Rabbit SAH models were duplicated by double injection of occipital accompaniment. VOL 500 mg·kg -1 and Nim 6 mg·kg -1 were administered orally once every 8 hours. After 5 days of treatment, they were shown by histochemical methods. In basilar artery and hippocampal CA1 microvessels of rabbits, the diameter of basilar artery and microvessel density were quantitatively analyzed by CMIAS image analysis system and stereological method. The histopathological changes in hippocampal CA1 region were observed, and VOL was evaluated for cerebral vasospasm after SAH. effect. RESULTS: The diameter of the basilar artery was significantly reduced at 5 days after SAH, and the length density and volume density of microvessels in the hippocampal CA1 region were significantly decreased. The neurons in the hippocampal CA1 region were degenerate and necrotic. The diameter of the basilar artery and microvessel density returned to normal after VOL treatment. Neurons were rare. Necrosis was significantly different from that in the SAH group, and its effect size was not significantly different from that of the Nim. Conclusion: VOL can dilate cerebral basilar artery, improve microcirculation and protect neurons. It is a Chinese herbal medicine worthy of further research and development.