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应用(32)~P标记人巨细胞病毒(HCMV)Towne株DNA EcoRI B克隆片段作为探针,在国内首次建立了早孕期产前诊断先天性HCMV感染的核酸分子杂交方法。该探针可检测到1.6pg同源DNA序列,与单纯疱疹病毒、EB病毒及人源性细胞DNA无杂交反应。在检测的150份绒毛样本中,10份杂交阳性,阳性率为6.67%。杂交法与病毒分离法相比较,杂交法的敏感性为100%,特异性为71.43%,准确性为86.67%。实验结果表明核酸分子杂交法为早孕期先天性HCMV感染的产前诊断提供了一项有效的手段。
Using DNA EcoRI B clone of human cytomegalovirus (HCMV) Towne strain (32) ~ P as a probe, a nucleic acid hybridization method for the prenatal diagnosis of congenital HCMV infection in early pregnancy was established for the first time in China. The probe can detect 1.6pg homologous DNA sequence, and herpes simplex virus, Epstein-Barr virus and human cell DNA hybridization reaction. Of the 150 samples tested, 10 were positive for hybridization and the positive rate was 6.67%. Hybridization method compared with the virus isolation method, the sensitivity of the hybridization method was 100%, specificity was 71.43%, accuracy of 86.67%. The experimental results show that nucleic acid hybridization provides an effective method for prenatal diagnosis of congenital HCMV infection in early pregnancy.