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目的：分离培养卵巢上皮性癌肿瘤浸润淋巴细胞（ＴＩＬ），探讨其生物学特性。方法：用酶法或机械法消化肿瘤组织后，在有ＩＬ２、ＰＨＡ或ＯＫＴ３ 条件下培养。ＴＩＬ表型分析采用间接免疫荧光法，细胞毒活性测定采用ＭＴＴ检测法。结果：分离培养２０ 份卵巢癌ＴＩＬ，１８ 份ＴＩＬ全部活化，另２份污染，最大扩增５２３倍。初分离的卵巢癌ＴＩＬ处于功能抑制状态（抑制期５～１５ｄ），对Ｋ５６２ 、Ｒａｊｉ和自体瘤细胞的细胞毒活性均很低，经体外培养活化增殖后，细胞毒活性明显增加，以特异性杀伤自体瘤细胞的能力增加最为明显。表型分析表明，ＴＩＬ体外培养过程中，ＣＤ３ 、ＣＤ８、ＣＤ２５（ＩＬ２Ｒ）和ＨＬＡＤＲ的表达率均明显上升。结论：卵巢癌ＴＩＬ体外培养后特异性杀伤自体瘤细胞的能力明显增加，可用于生物学治疗。
Objective: To isolate and culture epithelial ovarian tumor-infiltrating lymphocytes (TILs) and investigate their biological characteristics. Methods: After enzymatic or mechanical digestion of tumor tissue, with IL 2, PHA or OKT3 conditions. TIL phenotype analysis using indirect immunofluorescence, cytotoxicity assay using MTT assay. Results: 20 TILs were isolated and cultured, 18 TILs were all activated and the other 2 were contaminated. The maximum amplification was 523 fold. TIL was initially isolated from the ovarian cancer in a state of functional inhibition (inhibitory period of 5 ~ 15d), cytotoxic activity of K562, Raji and autologous tumor cells were very low, after in vitro culture activated proliferation, cytotoxic activity was significantly increased, with specificity The ability to kill autologous tumor cells increases most dramatically. Phenotypic analysis showed that the expression rates of CD3, CD8, CD25 (IL-2R) and HLA-DR in TIL in vitro were significantly increased. Conclusion: The ability of TIL in vitro to kill specific tumor cells in vitro is obviously increased, which can be used in biological treatment.