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目的研究Evi1基因特异性小干扰RNA(siRNA)对人红白细胞白血病(HEL)细胞增殖及生长周期的影响。方法将HEL细胞分为三组:Evi1基因siRNA组(A组)、siRNA-co对照组(B组)、细胞空白对照组(C组)。转染48 h后,台盼蓝染色实验检测细胞活力,流式细胞术检测细胞周期和凋亡率。结果与B、C组相比,A组HEL细胞活性下降[(97.13±1.58)%、(99.20±2.27)%vs.(26.05±2.49)%],细胞周期阻滞在G0/G1期[(39.61±1.32)%、(35.77±1.31)%vs.(79.07±1.43)%],S期细胞减少[(57.74±1.08)%(、57.36±1.38)%vs.(9.51±0.75)%],凋亡率增高[(9.78±1.14)%(、8.67±1.89)%vs.(49.94±1.75)%](P均<0.05);而B组和C组间各观察指标比较差异无统计学意义(P>0.05)。结论 Evi1基因特异性siRNA可抑制HEL细胞增殖,促进细胞凋亡。
Objective To investigate the effect of Evi1 gene-specific small interfering RNA (siRNA) on the proliferation and growth cycle of human erythroblastic leukemia (HEL) cells. Methods HEL cells were divided into three groups: Evi1 gene siRNA group (A group), siRNA-co control group (B group) and cell blank control group (C group). Forty-eight hours after transfection, trypan blue staining assay was used to detect cell viability. Flow cytometry was used to detect cell cycle and apoptosis rate. Results Compared with group B and group C, the activity of HEL cells in group A was decreased (97.13 ± 1.58% vs 99.20 ± 2.27% vs 26.05 ± 2.49% (57.36 ± 1.38)% vs (9.51 ± 0.75)%] in the S phase (39.61 ± 1.32% vs 35.77 ± 1.31% vs 79.07 ± 1.43%, respectively) (9.78 ± 1.14)% (, 8.67 ± 1.89)% vs (49.94 ± 1.75)%] (P all <0.05), while there was no significant difference between B group and C group (P> 0.05). Conclusion Evi1 gene-specific siRNA can inhibit the proliferation of HEL cells and promote apoptosis.