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本文研究了IL-2、IL-4、IL-6基因转染后B16黑色素瘤细胞表面MHCⅠ类抗原及ICAM-1的表达水平,并探讨了其在CTL诱导过程中的作用.结果表明,IL-2、IL-4、IL-6基因转染的B16黑色素瘤细胞表面MHCⅠ类抗原及ICAM-1表达均高于野生型B16黑色素瘤细胞及转染对照质粒的B16黑色素瘤细胞.体内接种后,小鼠脾脏CTL活性明显增强,CTL培养体系中IFN-γ、TNF-α的分泌水平也增高.在CTL诱导体系中加入抗ICAM-1单抗可以抑制CTL的活化,加入抗MHCⅠ类分子单抗后可使CTL的活化完全阻断.这提示细胞因子基因转染可能通过使肿瘤细胞表面MHCⅠ类抗原、ICAM-1的表达增加,从而增强瘤苗的免疫原性.
This study investigated the expression levels of MHC class I antigen and ICAM-1 on the surface of B16 melanoma cells after transfection of IL-2, IL-4, and IL-6 genes, and explored their role in CTL induction. The results showed that IL -2, IL-4, IL-6 gene transfected B16 melanoma cell surface MHC class I antigen and ICAM-1 expression were higher than wild-type B16 melanoma cells and B16 melanoma cells transfected with control plasmid. After inoculation in vivo The activity of CTL in the spleen of mice was significantly enhanced, and the secretion of IFN-γ and TNF-α in the culture system of CTL was also increased. The addition of anti-ICAM-1 mAb in the CTL induction system can inhibit the activation of CTL and the addition of anti-MHCI molecules. The activation of CTL can be completely blocked after anti-infection. This suggests that the transfection of cytokine genes may increase the expression of MHC class I antigen and ICAM-1 on the surface of tumor cells, thereby enhancing the immunogenicity of tumor vaccines.