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目的研究和探讨类风湿关节炎(RA)中葡萄糖-6-磷酸-异构酶(GPI)抗原的检测及其临床意义。方法用酶联免疫吸附试验(ELISA)分别对43例RA患者、20例其他自身免疫性疾病患者和36名健康人的血清进行GPI抗原检测,用免疫透射比浊法检测类风湿因子(RF)。结果GPI抗原在RA中有30例阳性,阳性率为70%,在其他自身免疫性疾病中有1例阳性,阳性率为5%,在健康人中全部阴性。RF在RA中有33例阳性,阳性率为77%,在其他自身免疫性疾病中有5例阳性,阳性率为25%,在健康人中有4名阳性,阳性率为11%。GPI抗原和RF对RA的特异性分别为98%和84%。GPI的敏感性与RF差异无统计学意义(P>0.05),而特异性显著高于RF(P<0.01)。结论GPI抗原是检测RA的特异性很高的血清学指标,可能成为诊断RA的一个新的实验室指标。
Objective To investigate and explore the detection of glucose-6-phosphate-isomerase (GPI) antigen in rheumatoid arthritis (RA) and its clinical significance. Methods Serum samples from 43 patients with RA, 20 patients with other autoimmune diseases and 36 healthy individuals were tested for GPI antigen by enzyme - linked immunosorbent assay (ELISA), and rheumatoid factor (RF) was detected by immunoturbidimetry. . Results The GPI antigen was found to be positive in 30 of RA patients, with a positive rate of 70%. Of the other autoimmune diseases, 1 was positive and the positive rate was 5%, all negative in healthy subjects. RF was found in 33 of 33 RA patients, with a positive rate of 77%. Of the other autoimmune diseases, 5 were positive, with a positive rate of 25%, and 4 were positive in healthy individuals with a positive rate of 11%. The specificity of GPI antigen and RF for RA was 98% and 84%, respectively. There was no significant difference between GPI sensitivity and RF (P> 0.05), but the specificity was significantly higher than RF (P <0.01). Conclusion GPI antigen is a highly specific serological marker for the detection of RA and may be a new laboratory marker for the diagnosis of RA.