Calculated results of inertia moment of turbo-generator rotor can be quite different by methods used in load rejection tests. In view of fluctuation features of rotor speed rise curve during load rejection tests, the measurement principle of rotor inertia moment was expounded. Based on the measured data in load rejection tests for an imported type of domestic 300-MW generating unit, the rotor speed rise curve was fitted with three kinds of functions to get initial runup rate, but the obtained results differed a lot from each other. According to analysis on the mechanism of rotor speed rise, m=2 consecutive points averaging or FFT (Fast Fourier Transform) smoothing technology was introduced to process test data, and then the initial runup rate was determined by the method of linear fitting of rotor speed in the range of governing valve closing time. Although the obtained curves had a fluctuating shape, the results of rotor inertia moment for 50% and 100% load rejection tests were of good consistency.