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目的:探讨c-k it受体及干细胞因子(stem cell factor,SCF)在子宫内膜异位症(Endom etriosis,EM s)患者的异位和在位内膜组织中的表达。方法:采用免疫组化SABC法检测32例EM s患者异位内膜(异位内膜组)及在位内膜组织(在位内膜组)c-k it和SCF的表达,并与30例非EM s患者在位内膜(对照组)进行比较。结果:①异位和在位内膜组中,c-k it阳性表达平均光密度(MOD)分别为0.42±0.09和0.37±0.07,两者比较差异无统计学意义(P>0.05),但均高于对照组的0.28±0.07(P<0.05)。②异位和在位内膜组中,SCF阳性表达分别为0.27±0.07和0.24±0.09,两者比较差异无统计学意义(P>0.05),均高于对照组的0.15±0.04(P<0.05)。③c-k it和SCF在异位内膜组增生期和分泌期比较差异无统计学意义(P>0.05);在在位内膜组和对照组的分泌期高于增生期(P<0.05);c-k it和SCF在位内膜组分泌期和增殖期的表达均高于对照组同期水平(P<0.05)。④c-k it和SCF在异位内膜组Ⅰ/Ⅱ期与Ⅲ/Ⅳ期中的表达差异无统计学意义(P>0.05)。结论:c-k it/SCF在内异症患者异位和在位内膜中的表达增强,提示c-k it/SCF可能在子宫内膜异位症的发生、发展中起重要作用。
Objective: To investigate the expression of c-kit receptor and stem cell factor (SCF) in ectopic and eutopic endometrium of patients with endometriosis (EMs). Methods: Immunohistochemical SABC method was used to detect the expression of ckit and SCF in ectopic endometrium (ectopic endometrium group) and eutopic endometrium (eutopic endometrium group) in 32 patients with EM s, EM s patients in endometrium (control group) were compared. Results: (1) In ectopic and eutopic endometrium groups, the mean optical density (ck) of ck it positive expression was 0.42 ± 0.09 and 0.37 ± 0.07 respectively, with no significant difference between the two groups (P> 0.05) 0.28 ± 0.07 in the control group (P <0.05). ② In ectopic and eutopic endometrium groups, the positive expression of SCF was 0.27 ± 0.07 and 0.24 ± 0.09 respectively, with no significant difference between the two groups (P> 0.05), which were all higher than that of the control group (0.15 ± 0.04, P < 0.05). There was no significant difference in the expression of c-kit and SCF between eutopic and ectopic endometrium (P> 0.05), but higher in eutopic endometrium and control group than in proliferative phase (P <0.05) ; ck it and SCF in eutopic endometrium secreting and proliferative phase expression were higher than the same period of the control group (P <0.05). ④ There was no significant difference in the expression of c-kit and SCF between stage Ⅰ / Ⅱ and stage Ⅲ / Ⅳ in ectopic endometrium (P> 0.05). Conclusion: The expression of c-kit / SCF in ectopic and eutopic endometrium of patients with endometriosis is increased, suggesting that c-kit / SCF may play an important role in the development of endometriosis.