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目的 探讨 p53基因突变在鼻腔、鼻窦恶性肿瘤发生发展中的突变状况及其意义。方法 用聚合酶反应-单链构象多态性分析(PCR-SSCP)方法测定36例鼻腔、鼻窦恶性肿瘤中p53基因4个突变热点,第5~8外显子。结果 鼻腔及鼻窦恶性肿瘤p53基因突变率为38.9%(14/36)与正常鼻粘膜组织相比,与慢性鼻炎粘膜组织相比有显著性差异(x2=11.874,P<0.05)。淋巴结转移阳性组突变率为66.7%(10/15),阴性组突变率为19%(4/21),两者相比有显著性差异(X2=10.219,P<0.05)但与临床分期、病理分型无关。结论 p53基因突变可能在鼻腔、鼻窦恶性肿瘤发生发展过程中是一个重要的分子事件,淋巴结转移和晚期有上升趋势。
Objective To investigate the mutation status of p53 gene mutation in the development of nasal and nasal sinus malignancies and its significance. Methods Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method was used to detect the mutations of p53 hot spot and exon 5 to exon in 36 cases of nasal and nasal sinus cancer. Results The mutation rate of p53 gene in nasal and sinus malignant tumors was 38.9% (14/36), which was significantly lower than that in normal nasal mucosa (x2 = 11.874, P <0.05). The positive rate of lymph node metastasis was 66.7% (10/15), and the negative rate was 19% (4/21) (P <0.05) Pathological type has nothing to do. Conclusion The mutation of p53 gene may be an important molecular event in the development of malignant tumors of the nasal cavity and nasal cavity. The incidence of lymph node metastasis and the up-regulation of p53 gene are up-regulated.