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本文报道以复旦大学遗传所863计划研究成果高蛋白转基因马铃薯试管苗为材料,选用含有大量元素、微量元素、糖源、碳源和渗透压稳定剂、激素的20个培养基配方,在实验室进行快速繁殖。试验结果表明,无论是液体培养还是固体培养,能显著促进茎长和分枝数增加的为9号培养基,促根系生长的为10号培养基,诱导薯块形成的为14号培养基,而液体培养基优于固体培养基。因此可以认为,在液体培养基中加入0.1～1毫克/升6—BA和0.15%活性炭可以提高试管苗的繁殖系数。加入0.5～1毫克/升6—BA和0.15%活性炭能诱导试管薯形成。
In this paper, Fudan University Institute of Genetics 863 Research High Protein Genetically Modified Potato Plantlets as material, selection of a large number of elements, trace elements, sugar, carbon source and osmotic pressure stabilizer, hormone 20 medium formulations in the laboratory Rapid breeding. The results showed that either liquid culture or solid culture can significantly promote the growth of stems and branches of the No. 9 medium to promote the root growth of No. 10 medium to induce the formation of tubers for the 14th medium, Liquid medium is superior to solid medium. Therefore, it can be considered that adding 0.1-1 mg / L 6-BA and 0.15% activated carbon into the liquid medium can increase the multiplication coefficient of test-tube seedlings. Tube formation was induced by the addition of 0.5-1 mg / L 6-BA and 0.15% activated carbon.