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目的:研究益肾康颗粒对糖尿病肾病(Diabetic Nephropathy,DN)大鼠模型尿微量白蛋白(MA)及肾脏病理形态的影响,并观察不同浓度益肾康颗粒对糖尿病肾病的治疗作用。方法 :SPF级SD雄性大鼠120只,随机选取108只大鼠,采用腹腔注射链脲佐菌素(Streptozotocin,STZ)53 mg/kg诱导大鼠糖尿病肾病模型,成膜后随机分为模型组、中药高、中、低剂量组及西药组。8周末取尿测定尿微量白蛋白;8周末大鼠麻醉后摘取左肾从中切开,置10%福尔马林中固定,制作光镜标本。结果 :光镜下观察中药组、西药组肾小球形态、大小、系膜基质较模型对照组损伤轻微且中药组优于西药组;尿微量白蛋白测定中药高、中、低剂量组及西药组与模型对照组比较具有统计学意义(P<0.05)。结论 :中药益肾康颗粒及西药雷米普利对早期糖尿病肾病均具有明显的治疗作用且中药组优于西药组。
Objective: To investigate the effects of Yishenkang granule on urinary microalbumin (MA) and renal pathological changes in diabetic nephropathy rats and to observe the therapeutic effects of Yishenkang granules on diabetic nephropathy. Methods: 120 Sprague-Dawley (SD) male Sprague-Dawley rats were randomly divided into model group (n = 108) and diabetic rats model induced by streptozotocin (STZ) 53 mg / kg , Chinese medicine high, medium and low dose group and western medicine group. Urinary microalbuminuria was measured on the 8th weekend. After the rats were anesthetized at the 8th week, the left kidney was excised and fixed in 10% formalin to prepare light microscopy specimens. Results: Under light microscopy, the glomerular morphology, size and mesangial matrix of the TCM group and the western medicine group were less damaged than the model control group and the Chinese medicine group was better than the Western medicine group; the microalbuminuria of the Chinese medicine group and the western medicine group were lower than those of the western medicine group Group and model control group was statistically significant (P <0.05). Conclusion: Chinese medicine Yishenkang granules and western medicine ramipril have obvious therapeutic effects on early diabetic nephropathy, and the traditional Chinese medicine group is superior to western medicine group.