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2.0 Gy X射线照射雄鼠不同发育阶段的生殖细胞与超数排卵处理的正常雌鼠交配,制备受精卵染色体标本。结果表明;雄性原核染色体畸变量以精细胞>成熟精子>精母细胞>精原细胞的顺序发生,其中前二者与自身正常对照比较,差异非常显著(p<0.01),与国外有关报道相一致。照后170d的精原细胞易位,明显高于正常对照,而且与照后140 d观察的显性致死突变明显相关。[实验证实用受精卵技术检测减数分裂后染色体损伤是良好的实验手段,而观察精原干细胞易位,用精母细胞实验更理想。
Germ cells of different developmental stages of 2.0 Gy X-ray irradiation were mated with normal female rats subjected to superovulation treatment to prepare fertilized egg chromosomes. The results showed that male prokaryotic chromosomal aberrations occurred in the order of sperm cells>mature spermatozoa>spermocytes>spermatogonia. The difference between the former two and their normal controls was significant (p<0.01). Consistent. The spermatogonia translocation at 170 days after irradiation was significantly higher than that of the normal control and was significantly correlated with the dominant lethal mutation observed 140 days after irradiation. [Experimental verification of the use of fertilized egg technology to detect chromosomal damage after meiosis is a good experimental method, and observation of spermatogonial stem cell translocation, spermatocyte test is more ideal.