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A new species of the genus Tylototriton is described in this article based on morphological and genetic evidence.The new species is identified belonging to the T.asperrimus group and is similar to T.wenxianensis.The diagnostic characters of the new species are as follows:dorsal ridge broad and thick,its width approximately equal to eye diameter (P ＜ 0.001); tail height greater than width at base of tail (P ＜ 0.001); no villous genital papilla found inside the male anal fissure; nodule-like warts,along lateral margin of the trunk,bulge and forming tubercles,and thin and transverse striae present between the tubercles.