论文部分内容阅读
目的建立参松养心胶囊中4种主要成分芍药苷、马钱苷、小檗碱、丹酚酸B的HPLC含量测定方法。方法采用RP-HPLC法,色谱柱为Agilent Extend C18(250 mm×4.6 mm,5μm);流动相为0.18%甲酸甲醇溶液-0.30%甲酸水溶液梯度洗脱;流速为1 mL/min;检测波长为230 nm;柱温为35℃。结果芍药苷、马钱苷、小檗碱、丹酚酸B分别在0.401 6～1.405 6μg、0.161 6～0.565 6μg、0.329 6～1.153 6μg、0.644 0～2.254 0μg的范围内线性关系良好;平均回收率分别为100.18%、102.92%、99.03%和98.25%,RSD分别为1.51%、1.61%、2.12%和2.24%。结论 10批样品测定结果表明,该方法简便、准确,可用于参松养心胶囊中4种主要成分的含量测定。
Objective To establish a HPLC method for the determination of paeoniflorin, loganin, berberine and salvianolic acid B, the main constituents of Shensong Yangxin Capsule. Methods The mobile phase consisted of an Agilent Extend C18 (250 mm × 4.6 mm, 5 μm) column with gradient elution of 0.18% formic acid in methanol and 0.30% formic acid in water with a flow rate of 1 mL / min. The detection wavelength was 230 nm; column temperature 35 ℃. Results Paeoniflorin, loganin, berberine and salvianolic acid B had good linearity in the range of 0.401 6 ~ 1.405 6μg, 0.161 6 ~ 0.565 6μg, 0.329 6 ~ 1.153 6μg and 0.644 0 ~ 2.254 0μg, respectively. The average recovery Rates were 100.18%, 102.92%, 99.03% and 98.25%, respectively, with RSDs of 1.51%, 1.61%, 2.12% and 2.24%, respectively. Conclusion The results of 10 batches of samples show that the method is simple and accurate and can be used to determine the content of four main components in Shensongyangxin Capsule.