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我们于1967年开展了紫云英根瘤菌的分离筛选工作,从中获得粉红色菌株,代号“红11”。其性能较好,在生产上已得到应用。新选的紫看英根瘤菌,具有两个显著特点:一是菌落呈粉红色;二是血清度应均不同于现有的参试菌株,是一个新的血清型。这个有色紫云英根瘤菌的获得,对于发掘固氮微生物新资源有着现实意义。其有色标志,在生产上可作为菌落生长指示,并在研究遗传工程中也可加以利用。菌种分离我们在紫云荚现蕾、初花、盛花三个时期选长势好的植株,取主根上粉红色的大、中形根瘤100多个作为分离材料。要求在24小时内进行培养分离。培养基分二种:
In 1967, we carried out the isolation and screening of Rhizobium japonicum, and obtained the pink strain “Red 11” from it. Its performance is better, has been applied in production. The newly-selected Rhizobium purple look, has two salient features: First, the colony is pink; Second, the serum should be different from the existing test strains, is a new serotype. The obtainment of this colored astragalus rhizobium has practical significance for discovering new resources of nitrogen fixation microorganisms. Its colored logo, which is used as an indication of colony growth in production, can also be used in the study of genetic engineering. Isolation of bacteria in the purple cloud pod buds, first flower, full bloom three periods of growing good plants, take the main root of pink large, medium-sized root nodules more than 100 as a separation material. Requires culture within 24 hours of separation. Medium divided into two kinds: