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白藜芦醇是一种具有抗肿瘤、抗衰老等医疗和保健功能的非黄酮类多酚化合物,在天然植物中含量极低。目前采用化学法合成白藜芦醇需要使用有毒催化剂且会产生大量副产品。在构建成功大肠杆菌代谢工程菌基础上,文章着重报道了重组大肠杆菌培养条件和生物转化合成白藜芦醇条件的优化研究。研究表明,将在YM9培养基中培养10 h后经离心获得的重组菌体进一步重悬于含有1.2 g/L对香豆酸和聚乙二醇的YM9培养基中,在优化条件下24 h培养液经乙酸乙酯萃取经液相测定白藜芦醇的产量达到598 mg/L。此研究为利用重组大肠杆菌大规模生物合成白藜芦醇打下了基础。
Resveratrol is a non-flavonoid polyphenolic compound with anti-tumor, anti-aging and other medical and health care functions. It is very low in natural plants. The current chemical synthesis of resveratrol requires the use of toxic catalysts and produces large amounts of by-products. Based on the successful construction of Escherichia coli metabolic engineering bacteria, the article focuses on the optimization of the conditions of recombinant Escherichia coli and biotransformation into resveratrol. The results showed that the recombinant bacteria obtained after centrifugation in YM9 medium for 10 h were further resuspended in YM9 medium containing 1.2 g / L p-coumaric acid and polyethylene glycol, and under optimal conditions for 24 h The medium was extracted with ethyl acetate and the yield of resveratrol was determined to be 598 mg / L. This study laid the foundation for the large-scale biosynthesis of resveratrol by recombinant Escherichia coli.