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[目的]探索Cu2+和Zn2+胁迫对紫花苜蓿种子萌发、根长及植株生长的影响。[方法]重金属胁迫分别设置9个浓度梯度:Cu2+为0、10、25、50、80、100、200、300和400 mg/L;Zn2+为0、50、100、150、200、300、400、500、600 mg/L,利用组织培养方法,研究Cu2+和Zn2+胁迫对紫花苜蓿种子萌发及生长的影响。[结果]低浓度Cu2+≤25 mg/L、Zn2+≤100 mg/L对紫花苜蓿的萌发率、根长和株高生长具有促进作用,并且在Cu2+为10 mg/L、Zn2+为100 mg/L时根长和植株生长达到最佳。随着Cu2+、Zn2+浓度的增加,Cu2+浓度≥50 mg/L,Zn2+≥150 mg/L时对紫花苜蓿萌发率、根系和植株生长抑制作用逐渐加大,高浓度时Zn2+浓度为600 mg/L、Cu2+浓度为400 mg/L时,抑制作用最明显。[结论]不同Zn2+和Cu2+浓度对紫花苜蓿种子的萌发率、根长和植株生长有不同影响作用。
[Objective] The research aimed to explore the effects of Cu2 + and Zn2 + stress on alfalfa seed germination, root length and plant growth. [Method] Nine concentration gradients were set up for heavy metal stress respectively: Cu2 + 0,10,25,50,80,100,200,300 and 400 mg / L; Zn2 + 0,50,100,150,200,300,400 , 500 and 600 mg / L respectively. The effects of Cu2 + and Zn2 + stress on seed germination and growth of alfalfa were studied by tissue culture method. [Result] The results showed that Cu2 + ≤25 mg / L and Zn2 + ≤100 mg / L could promote the germination rate, root length and plant height of alfalfa at low concentrations of Cu2 + 10 mg / L and Zn2 + 100 mg / L When the root length and plant growth to achieve the best. With the increase of Cu2 + and Zn2 + concentration, Cu2 + concentration of 50 mg / L and Zn2 + 150 mg / L had a significant effect on the germination rate of alfalfa and the growth inhibition of roots and plants. The concentration of Zn2 + was 600 mg / L , Cu2 + concentration of 400 mg / L, the most obvious inhibition. [Conclusion] Different Zn2 + and Cu2 + concentrations had different effects on the germination rate, root length and plant growth of alfalfa seeds.