论文部分内容阅读
目的：观察犬冠脉发生周期性血流减少（ＣＦＲｓ）期间血小板α颗粒膜蛋白１４０（ＧＭＰ１４０）及ＴＸＢ２的变化，分析ＧＭＰ１４０在诊断ＣＦＲｓ中的意义．方法：开胸分离犬冠脉前降支（ＬＡＤ）２～３ｃｍ用血管钳钳夹方法损伤冠脉内膜后放置血管缩窄环，使冠脉至临界狭窄，缩窄环近端置电磁流量计，诱发ＣＦＲｓ．在诱发ＣＦＲｓ前及ＣＦＲｓ出现后４０ｍｉｎ冠状窦采血，用放免方法测ＧＭＰ１４０及ＴＸＢ２血浆浓度．结果：ＣＦＲｓ出现后４０ｍｉｎ，ＧＭＰ１４０及ＴＸＢ２显著升高［ＧＭＰ１４０从（４．２９±０．９９）μｇ／Ｌ升至（６．８２±１．１２）μｇ／Ｌ，Ｐ＜０．０１；ＴＸＢ２从（７５．６２±４１．８７）ｎｇ／Ｌ升至（１０９．９２±４５．９７）ｎｇ／Ｌ，Ｐ＜０．０１］；ＧＭＰ１４０与ＴＸＢ２有很好的正相关性（ｒ＝０．７３，Ｐ＜０．０１）．结论：ＧＭＰ１４０可能是ＣＦＲｓ中的一种重要介质，ＧＭＰ１４０有望成为诊断ＣＦＲｓ的一个较特异的指标．
Objective: To observe the changes of platelet α-granule membrane protein 140 (GMP140) and TXB2 during periodontal blood flow reduction (CFRs) in dogs, and to analyze the significance of GMP140 in the diagnosis of CFRs. Methods: Thoracotomy was performed to separate the coronary artery from the critical stenosis by narrowing the blood vessel narrowing ring and then narrowing the electromagnetic flux near the proximal end of the coronary artery Count, induce CFRs. Coronary sinusoids were collected 40 minutes before and after CFRs were induced, and plasma concentrations of GMP140 and TXB2 were measured by radioimmunoassay. Results: The levels of GMP140 and TXB2 were significantly increased at 40 min after CFRs appeared (GMP140 increased from (4.29 ± 0.99) μg / L to (6.82 ± 1.12) μg / L, P <0.01) From (75.62 ± 41.87) ng / L to (109.92 ± 45.97) ng / L, P <0.01]. There was a positive correlation between GMP140 and TXB2 (r = 73, P <0.01). Conclusion: GMP140 may be an important mediator in CFRs. GMP140 is expected to be a more specific indicator for the diagnosis of CFRs.