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FOXQ1是FOX家族的的重要成员之一,其参与了多种人类肿瘤的上皮间质转化(epithelialmesenchymal transition,EMT).本研究设计合成了FOXQ1基因的shRNA(short hairpin RNA),用此转染SW480细胞,通过显微镜观察细胞形态,Transwell小室、细胞黏附试验检测转移能力及黏附能力,以探索FOXQ1与结直肠癌细胞EMT的关系.结果显示,沉默FOXQ1后,SW480细胞顶底极性及细胞间紧密连接增加,侵袭、迁移的细胞数目减少,同种黏附能力增加,异种黏附能力降低.进一步的机制研究表明,沉默FOXQ1基因可以导致SW480细胞的上皮标志因子E-cadherin表达显著增高,而间质细胞标志因子N-cadherin、Vimentin及MMP2表达均降低.以上结果表明,沉默FOXQ1基因可以逆转SW480细胞EMT,其机制可能与E-cadherin的上调和N-cadherin、Vimentin、MMP2的下调有关,这为进一步研究FOXQ1在结直肠癌发生发展中的作用提供实验基础.
FOXQ1 is one of the important members of the FOX family and is involved in the epithelialmesenchymal transition (EMT) of many human tumors.In this study, short hairpin RNA (FOXQ1) was synthesized and transfected into SW480 Transwell chamber and cell adhesion assay were used to detect the metastatic ability and adhesive ability of FOXQ1 in colorectal cancer cells to explore the relationship between FOXQ1 and EMT.Results showed that after silencing FOXQ1, the polarity of SW480 cells and the intercellular tightness The number of cells with increased, invasion and migration decreased, alloadhesive ability increased, and xeno-adhesion ability decreased.Further mechanism studies showed that silencing FOXQ1 gene could significantly increase the expression of E-cadherin, an epithelial marker of SW480 cells, while stromal cells The results indicated that silencing FOXQ1 could reverse the EMT of SW480 cells, which may be related to the up-regulation of E-cadherin and the down-regulation of N-cadherin, Vimentin and MMP2, To study the role of FOXQ1 in the development of colorectal cancer provide experimental basis.