【摘 要】
：
With ion implantation (N+, energy 10 keV and dosage 1.56×1015N+ cm-2), a high xylanase-producing strain Aspergillus niger N212 was selected. Based on an orthog
【机 构】
：
Key Laboratory of Ion Beam Bioengineering of Institute of Plasma Physics
【基金项目】
：
the National Key Technologies R & D Program of China during the 10th Five-Year Plan Period
论文部分内容阅读
With ion implantation (N+, energy 10 keV and dosage 1.56×1015N+ cm-2), a high xylanase-producing strain Aspergillus niger N212 was selected. Based on an orthogonal experiment, an optimal fermentation condition was designed for this high-yield strain. The suitable medium was composed of 8% corncob; 1.0% wheat bran; 0.1% TWEEN20; 0.5% (NH4)2SO4;3.0×10-4. At present, under our experiment condition, xylanase activity of Aspergillus niger N212 reached a level of 600 IU/ml, almost increased by 100% in xylanase production and the time of yielding xylanase was largely reduced to 12 h at 28 ℃.
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