论文部分内容阅读
本文以差异蛋白质组学为理论研究基础,利用高效液相色谱串联质谱(HPLC-MS/MS)实现了对猪肉或鸡肉掺假的牛肉的定性鉴别及定量分析。首先采用高分辨质谱仪(nLC-QE)分别找出牛肉、猪肉和鸡肉的相对专属性多肽链,并对其进行二级扫描,得到了三个物种的特异性离子对,进一步利用HPLC-MS/MS的多反应监测模式(MRM)对掺杂猪肉或鸡肉的牛肉进行特异性离子对验证,分别选择了牛肉、猪肉和鸡肉的9、8和7条多肽进行定性研究;并与聚合酶链式反应(PCR)技术进行比对,得到的结果一致;此外将鸡肉和猪肉以0.5、1.0、5.0、10.0、25.0%的比例掺杂于牛肉中,各选择3组离子对进行定量分析,其各自的线性相关系数均大于0.99,最低定量限为0.5%。由此表明,液相色谱串联质谱的方法可以快速地对牛肉中的猪肉和鸡肉进行鉴别并能准确定量分析。
In this paper, differential proteomics as the theoretical basis for the study, the use of high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS) to achieve the qualitative identification of pork or chicken adulterated beef and quantitative analysis. Firstly, nLC-QE was used to identify the relative specific polypeptide chains of beef, pork and chicken, respectively. The three-species specific ion pairs were obtained by two-step scans. By using HPLC-MS / MS Multiple Reaction Monitoring Model (MRM) was used to verify the specificity of ion-pair for beef with or without pork, and 9, 8 and 7 peptides of beef, pork and chicken were selected for qualitative study. (PCR) technique, the results were consistent. In addition, chicken and pork were dipped in beef at 0.5, 1.0, 5.0, 10.0 and 25.0% respectively. Three groups of ions were selected for quantitative analysis. Their respective linear correlation coefficients were greater than 0.99, the lowest limit of quantification was 0.5%. Thus, the method of tandem mass spectrometry of liquid chromatography can rapidly identify pork and chicken in beef and can accurately and quantitatively analyze the same.