Epon-812包埋剂是目前电镜制样中较普遍采用的一种优良包埋剂.它具有许多优点,如切片性能好,超薄切片结构清晰,耐电子束轰击,特别是捞片时铜网无需做支持膜,为切片人员提供了方便,如果一次切片未捞好,可以再浸入水槽重新捞取,切片不会发生皱折.但是它也存在着一些不足:容易吸潮,对操作条件要求较高,性能不够稳定,尤其是刚开始使用时会遇到一些困难.我们使用该包埋剂多年,逐渐摸索到一些规律,主要有以下几点体会:1 试剂要成套采购.试剂成套与否直接决定了包埋剂的稳定性,如不成套,起码也应使用同一公司的产品,否则包埋剂的配方要重新调配,重新摸索,调配不当的包埋剂做出的包埋块,修块时发涩,而且毛糙,造成切薄片困难.2 试剂要保证新鲜.最理想的是一年以内生产的试剂,现买现用,这样的试剂配制出的包埋剂,做成包埋块后抗水性能强,即使包埋块丢入水中再取出,切片也不会有困难.3 试剂要保持干燥.NMA、DDSA均为酸酐,吸水能力很强,暂时不用的试剂要保存在干燥器内,使用的药品则分装成小包装放在操作箱内.4 操作条件要恒定.用Epon-812包埋时,影响因素最大的就是湿度,相对湿度超过35%以上,会出现聚合不均匀或聚合不完全,而聚合不好直接影响切片质量.上海地区湿度比较大,因此我们用Epon-812包埋的全过程均在操 Epon-812 embedding agent is an excellent embedding agent commonly used in electron microscopy sample preparation, and has many advantages such as good slicing performance, clear slice structure, electron beam bombardment resistance, especially copper The net does not need to do the support film, which provides convenience for the slicing personnel. If the slicing is not done well, it can be re-dipped into the sink to make the slicing without wrinkling. But it also has some disadvantages: it is easy to absorb moisture and requires operating conditions High, the performance is not stable enough, especially when it first encountered some difficulties encountered.We use the embedding agent for many years, and gradually found some laws, the main points are as follows: 1 Reagent to complete sets of procurement. Directly determines the stability of the embedding agent, if not complete, at least should also use the same company’s products, or embedding agent formulations to be redeployed, re-exploration, the deployment of improper embedding agent embedding blocks, repair Block astringent, and rough, resulting in thin slices difficult .2 reagents to ensure fresh. The ideal is produced within one year of reagents, buy now, such agents formulated out of embedding agents, made of embedded blocks Water resistance after strong, even if buried Thrown into the water and then removed, there will be no difficulty slicing .3 reagents to keep dry .NMA, DDSA are anhydride, strong absorbent capacity, temporary reagents to be stored in the dryer, the use of drugs is sub-small Packaging on the operation box .4 operating conditions to be constant .Embedded with Epon-812, the most influential factor is the humidity, relative humidity above 35%, there will be uneven polymerization or polymerization, and polymerization is not direct Affect slice quality.Shanghai humidity is relatively large, so we embed the whole process with Epon-812 are in operation