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本文采用免疫组化法对治疗量及中毒量吗啡注射大鼠死后体内吗啡的定位及分布变化分别进行研究。发现：⑴吗啡广泛分布于大脑皮质、海马回、基底核、丘脑、脑干、扣带回、嗅束、胼胝体及小脑神经细胞胞浆内及神经纤维；心、肝、肾、睾丸及血管平滑肌等也有吗啡分布，但胸腺、淋巴结等未发现吗啡存在；⑵治疗量吗啡注射鼠死后９６ｈ内，心、肝、肾、肾上腺等大部分实质器官组织细胞中吗啡含量随死后时间延长而明显减少（Ｐ＜０．０１）；中毒量吗啡注射鼠死后１２ｈ内，组织细胞中吗啡含量变化甚微，但死后２４－９６ｈ，吗啡量缓慢递减（Ｐ＜０．０５）。骨骼肌吗啡量随死后时间延长而明显增加，死后４８ｈ内脑组织吗啡量无明显变化。本研究证实死后尸体内吗啡再分布的客观存在，探讨了吗啡再分布发生机制。免疫组化法为海洛因或吗啡成瘾及中毒死亡的医学检测提供了新的手段。
In this paper, immunohistochemistry was used to study the morphological changes of morphine in post-mortem rats treated with morphine and the therapeutic dose of morphine. Found: ⑴ morphine is widely distributed in the cerebral cortex, hippocampus, basal ganglia, thalamus, brainstem, cingulate gyrus, olfactory tract, corpus callosum and cerebellar neurons cytoplasm and nerve fibers; heart, liver, kidney, testis and vascular smooth muscle Morphine distribution, but no morphine in the thymus, lymph nodes and other found; ⑵ treatment of morphine injection within 96h after injection, morphine, morphine, morphine, morphine, morphine, (P <0.01). Morphine content in tissue cells changed little within 12h after poisoning morphine injection, but gradually decreased from 24-96h after death (P <0.05). The amount of morphine in skeletal muscle significantly increased with the prolongation of post-mortem time, and there was no significant change in the amount of morphine in brain tissue within 48 hours after the death. This study confirmed the objective existence of post-mortem morphine redistribution and discussed the mechanism of morphine redistribution. Immunohistochemistry provides a new means of medical testing for heroin or morphine addiction and death from poisoning.