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通过单细胞凝胶电泳实验研究了不同浓度莫能菌素暴露对赤子爱胜蚓(Eisenia fetida)体腔细胞DNA的损伤,结果显示,50mg.kg-1莫能菌素处理组尾部DNA含量值最大、尾长值最大、Olive尾矩值最大,分别为34.539%、107.736μm和29.354;随着莫能菌素暴露剂量的增加,尾部DNA含量、Olive尾矩和尾长损伤频率增加;尾部DNA含量对莫能菌素暴露最为敏感,对照组和各处理组的尾部DNA含量之间均存在显著性差异(P<0.05);对照组与15、25、50 mg.kg-1处理组的Olive尾矩和尾长损伤频率之间均存在显著性差异(P<0.05);暴露浓度与尾部DNA含量、Olive尾矩和尾长具有良好的剂量-效应关系(P<0.05)。实验结果表明,蚯蚓体腔细胞DNA损伤可作为指示莫能菌素影响的生物标志物,彗星试验是检测莫能菌素暴露对赤子爱胜蚓活体基因损伤的有效手段。
Single cell gel electrophoresis was used to study the DNA damage induced by different concentrations of monensin exposure to Eisenia fetida. The results showed that the DNA content in the tail of 50 mg.kg-1 monensin treatment group was the largest , The tail length was the largest, and the Olive tail moment was the largest (34.539%, 107.736μm and 29.354, respectively). The tail DNA content, Olive tail moment and tail length damage frequency increased with the increase of monensin exposure dose. The tail DNA content The most sensitive to monensin exposure, there was a significant difference (P <0.05) between the tail DNA content of the control group and each treatment group; the control group and the 15, 25, 50 mg.kg-1 treatment group Olive tail (P <0.05). There was a good dose-effect relationship between the tail DNA content, Olive tail moment and caudal long tail length (P <0.05). The experimental results show that DNA damage in the body cavity of earthworms can be used as a biomarker to indicate the effect of monensin. Comet assay is an effective measure to detect the gene damage caused by monensin exposure in vivo of Eisenia.