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目的 1,2 5 (OH) 2 D3 、钙代谢紊乱是慢性肾衰继发性甲状旁腺功能亢进症 (SHPT)的主要原因 ,它们分别与维生素D受体 (VDR)、2 8ku钙结合蛋白 (CaBP D2 8k)结合而发挥作用 ,通过观察 5 / 6肾切除大鼠残余肾VDR、CaBP D2 8k表达 ,探讨其在早期SHPT中的作用。方法 采用免疫组化ABC法观察 5 / 6肾切除大鼠残余肾VDR、CaBP D2 8K蛋白阳性表达。结果 慢性肾功能衰竭 (CRF)大鼠血清全段甲状旁腺素水平增高 ,肾小管CaBP D2 8K阳性率明显增加 ,而VDR表达无明显改变。结论 早期CRF已有SHPT表现 ,残余肾CaBP D2 8K表达增强可能为纠正CRF钙、磷代谢紊乱的代偿机制之一。
Objective 1,25 (OH) 2 D3, a disorder of calcium metabolism, is the main reason of secondary hyperparathyroidism (SHPT) in chronic renal failure. They are associated with vitamin D receptor (VDR), 28 kD calcium-binding protein (CaBP D2 8k). The effect of VDR and CaBP D2 8k on the expression of VDR and CaBP D2 8k in remnant kidney of 5/6 nephrectomized rats was observed. Methods Immunohistochemical ABC method was used to observe the expression of VDR and CaBP D2 8K protein in 5/6 nephrectomized rats. Results The level of parathyroid hormone increased in the serum of chronic renal failure (CRF) rats and the positive rate of CaBP D2 8K in renal tubules increased significantly, while the expression of VDR did not change significantly. Conclusion The early stage of CRF has SHPT performance, and the enhanced expression of residual CaBP D2 8K may be one of the compensatory mechanisms to correct the disorder of calcium and phosphorus metabolism of CRF.