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利用根癌农杆菌介导的遗传转化,将启动子诱捕(Promotertrapping)元件插入到棉花基因组,获得141个独立的转化子,其中97%的转化子经PCR扩增为阳性。不同组织中GUS基因的表达频率为:根部48%,茎的微管组织9.2%,叶5.2%,花51%;同时检测了不同植株中GUS基因的表达模式,发现GUS基因在不同株系的植株间的表达模式呈现较大的差异,有些植株中GUS基因是组织特异表达,有些则是器官特异表达,有些则在多个器官中均有表达。所建立的启动子诱捕系统中的GUS基因高频率、多模式和时空特异性表达为分离基因及其调节序列、开展功能基因组研究奠定了坚实的基础。
Using Agrobacterium tumefaciens-mediated genetic transformation, the promoter Promotertrapping element was inserted into the cotton genome to obtain 141 independent transformants, of which 97% of the transformants were positive by PCR amplification. The expression frequency of GUS gene in different tissues was 48% in roots, 9.2% in stems, 5.2% in leaves and 51% in flowers. The expression patterns of GUS gene in different plants were also detected. The expression patterns among plants show great differences. In some plants, GUS gene is tissue-specific, while others are organ-specific, while others are expressed in multiple organs. The established GUS gene in promoter trapping system has high frequency, multimodal and spatiotemporal expression, which has laid a solid foundation for the isolation of genes and their regulatory sequences and the development of functional genomics.