论文部分内容阅读
目的:进一步探讨微小病毒B19(HPVBl9)与小儿川崎病(KD)发病的关系。方法:应用巢式PCR法和ELISA法对40例川崎病患儿(观察组)及30例随机挑选门诊健康体检儿童(对照组)进行B19-DNA检测和B19VP2IgM检测。对观察组KD患儿中HPVB19-DNA检测阳性的与阴性的两组中应用心脏彩超检测冠状动脉内径值变化进行比较。结果:观察组B19-DNA阳性检出率为27.5%(11/40),对照组B19-DNA检测均为阴性,两组比较差异有统计学意义(P<0.01)。观察组B19VP21gM阳性检出率为27.5%(11/40),对照组均阴性,两组比较差异有统计学意义(P<0.01)。B19-DNA和B19VP2IgM一致率为100.0%。40例观察组中HPVB19-DNA检测阳性的与阴性的两组中,心脏彩超冠状动脉内径值变化差异有统计学意义(P<0.01)。40例川崎病患儿在性别、年龄、常见临床表现、预后等方面无差别。结论:HPVB19与小儿川崎病发病有关,HPVB19可能是小儿川崎病病原之一;HPVB19与冠状动脉扩张或瘤形成可能相关,HPVB19感染所致冠状动脉扩张程度较重。
Objective: To further explore the relationship between parvovirus B19 (HPVBl9) and childhood Kawasaki disease (KD). Methods: B19-DNA and B19VP2 IgG were detected by nested PCR and ELISA in 40 children with Kawasaki disease (observation group) and 30 children (control group) randomly selected from outpatients. In the observation group KD children with HPVB19-DNA test positive and negative in both groups, the application of echocardiography to detect changes in coronary artery diameter values were compared. Results: The positive rate of B19-DNA in the observation group was 27.5% (11/40). The B19-DNA in the control group was negative. There was significant difference between the two groups (P <0.01). The positive rate of B19VP21gM in the observation group was 27.5% (11/40), and the negative rate in the control group was statistically significant (P <0.01). The concordance rate of B19-DNA and B19VP2IgM was 100.0%. In the 40 cases of observation group, there was significant difference in the diameter of coronary artery between the positive and negative groups of HPVB19-DNA (P <0.01). 40 cases of children with Kawasaki disease in gender, age, common clinical manifestations, prognosis, etc. no difference. Conclusions: HPVB19 is associated with the pathogenesis of Kawasaki disease in children. HPVB19 may be one of the pathogens of Kawasaki disease in children. HPVB19 may be associated with dilatation or tumor formation of coronary arteries, and coronary artery dilatation caused by HPVB19 infection is more serious.