关于腊螟杆菌及苏芸金杆菌的利用研究

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腊螟杆菌对菜青虫的室内毒力测定显示:4、5令幼虫个体吞食孢子12×10~3个以上,死亡率达100%。对幼虫羣体所致死亡率如下:菌液浓度0.3%及0.5%为89.3—100%,杆菌与 DDT 混合(浓度0.2%+1/6万)或与敌百虫混合(浓度为0.2%+1/8万)死亡率都达100%。苏芸金杆菌液浓度0.3%时,菜青虫死亡率为93.3%;与 DDT 混合液(0.1%+1/6万)或与敌百虫混合液(0.1%+1/8万),死死率分别为87.5%及82.4%。田间毒效腊螟杆菌液浓度0.3%,死亡率为56.5—79.2%,与 DDT 或敌百虫混合喷施(浓度0.2%+1/6万),死亡率为65.5—93.5%。液剂毒效高于粉剂。对玉米螟的室内毒力测定(两种菌液浓度均为0.02—0.5%):腊螟杆菌液处理玉米叶幼虫口服死亡率为68.9—79.2%,苏芸金杆菌液为64.1—75.5%。田间毒效,在春玉米上,两种稈菌单纯菌剂(液剂或颗粒剂)浓度0.1—0.5%,与DDT 混合剂(液剂或颗粒剂)浓度0.1%+0.05%,蛀孔率一般比对照低2.2—31.5%。在夏玉米上腊螟稈菌单纯菌剂(浓度为0.2%),或与 DDT 合剂(0.1%+0.02%),蛙孔率比对照低1.4—17.0%,苏芸金稈菌几无效果。颗粒剂用菌量较液剂差4倍,而毒效与液剂相当。室内毒力测定,两种稈菌对粘虫、梨小食心虫效果很差,对(?)螬无效果。两种稈菌对家蚕健康都有影响,腊螟稈菌液(浓度0.01—0.5%)致死率达80—100%;苏芸金杆菌液(浓度0.1%及0.5%)致死率为55.8%及94.2%。两种稈菌对蜜蜂全无影响,稈菌能存活在蜂蜜内,但繁殖不明显。 Laboratory tests on virulence of Bacteroidea to Pieris rapae showed that 4,5 larvae swallowed 12 × 10-3 spores individually and the mortality rate reached 100%. Mortality to the larval population was as follows: 0.3% and 0.5% of the bacterial broth were 89.3-100%, the bacilli were mixed with DDT (0.2% + 1/60 000) or mixed with trichlorfon (0.2% 1/8 million) all have 100% mortality rates. When the concentration of Bacillus thuringiensis strain was 0.3%, the mortality of Pieris rapae was 93.3%; with DDT mixture (0.1% + 1/60000) or mixture with trichlorfon (0.1% + 1/80000) The rates were 87.5% and 82.4% respectively. In the field, the concentration of Culex pipiens pallens was 0.3% and the mortality rate was 56.5-79.2%. The mortality was 65.5-93.5% when mixed with DDT or trichlorfon (concentration 0.2% + 1/60000). Liquid toxicity is higher than powder. The indoor toxicity test to corn borer (the concentration of the two kinds of bacteria was 0.02-0.5%): The oral mortality rate of corn borer larvae was 68.9-79.2% and that of the Bacillus thuringiensis strain was 64.1-75.5%. Field toxicity, the spring corn, the two kinds of bacteria single fungus agent (liquid or granular) concentration of 0.1-0.5%, and DDT mixture (liquid or granular) concentration of 0.1% +0.05%, the rate of bore Generally lower than the control 2.2-31.5%. The results showed that the frog bore rate was 1.4-17.0% lower than that of the control on the corn with the simple fungus Bacillus cereus (concentration 0.2%) or DDT mixture (0.1% + 0.02%). Granules with bacteria than the liquid 4 times worse, while the toxic effects and liquid quite. Indoor toxicity test, two kinds of stalk bacteria on armyworms, pear borer is very poor, no effect on (?) 螬. Both strains of Staphylococcus aureus have an impact on the health of silkworm. The lethality of Bacillus subtilis (concentration 0.01-0.5%) is 80-100%; that of Bacillus thuringiensis (concentration 0.1% and 0.5%) is 55.8% 94.2%. Two kinds of stalks have no effect on bees, stalks can survive in the honey, but the reproduction is not obvious.
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