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本实验应用放射免疫方法，对雄性成年ＳＤ大鼠离体下丘脑、腺垂体、睾丸三级组织进行培养顺序灌注，观察Ｐ物质对于促性腺激素释放激素（ＧｎＲＨ）、黄体生成素（ＬＨ）及睾酮（Ｔ）分泌的影响。将实验动物５０只，随机分成五组。前三组进行下丘脑、腺垂体培养；后两组进行腺垂体、睾丸培养。将动物断头处死，在超静工作台上立即取出下丘脑组织（包括视前区和下丘脑中央基底部）腺垂体组织及一侧睾丸组织。分别装入各自对应的预盛有５ｍｌ新鲜完全１６４０液的培养板孔中，向其中第二组下丘脑培养液中加入１ｎｇＳＰ后，进行培养。条件：９５％Ｏ２、５％ＣＯ２；温度：３７Ｃ；ｐＨ：７．２—７．４，３小时后，将各组下一级培养液取出２ｍｌ待测。同时从上一级培养液中取出１ｍｌ与１ｍｌ新鲜完全１６４０液共同加入到下一级培养液中，并对第三、五组同时向下一级培养液加入１ｎｇＳＰ，再培养３小时后，取出各自的２ｍｌ培养液进行放免测定，与上一级取出液的测量值进行比较。实验结果：第一组ＬＨ含量增值为（４６．０８＋３．９１）ｍＩＵ／ｍｌ，第二组（６４．２９＋４．７７）ｍＩＵ／ｍｌ，第三组（２９．５０＋３．１３）ｍＩＵ／ｍｌ，第四组Ｔ含量增量为（３．９４＋０．５２）ｎｇ／ｍｌ?
In this experiment, we used radioimmunoassay to perfuse the hypothalamus, adenohypophysis and testis in male adult SD rats in order to observe the effects of substance P on the expression of GnRH, LH, Effects of Testosterone (T) Secretion. 50 experimental animals were randomly divided into five groups. The first three groups of hypothalamus, adenohypophyseal; the latter two groups of adenohypophyseal, testicular culture. Animals were decapitated and the hypothalamic tissues (including the preoptic area and central base of the hypothalamus) adenohypophyseal tissue and one side of the testis were removed immediately on an ultra-quiet bench. Respectively, into their respective wells pre-filled with 5 ml of fresh 1640-well complete well, to which a second group of hypothalamic medium was added 1 ngSP and then cultured. Conditions: 95% O2, 5% CO2; temperature: 37C; pH: 7.2-7.4, 3 hours later, the next group of culture medium was removed 2ml test. At the same time, remove 1ml and 1ml fresh completely 1640 liquid from the previous culture medium to the next culture medium, add 1ngSP to the third and fifth groups to the next culture medium at the same time, cultivate for another 3 hours, The respective 2 ml culture broth was subjected to radioimmunoassay and compared with the measurement value of the upper stage extract. The results showed that in the first group, the added value of LH was (46.08 + 3.91) mIU / ml, the second group (64.29 + 4.77) mIU / ml and the third group (29.50 + 3.13) Four groups T content increment (3.94 +0.52) ng / ml?