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目的:探讨切除修复交叉互补基因1(ERCC1)在原发性肝细胞癌(Hepatocellular carcinoma,HCC)组织中的表达情况及临床意义。方法:应用免疫组织化学方法检测226例HCC癌组织、65例HCC癌旁组织和17例非癌肝组织中ERCC1的表达情况,并分析其与HCC临床病理因素和预后的关系结果:肝癌组织中ERCC1阳性率为44.2%(100/226),显著高于癌旁组织及非癌正常肝组织中的阳性率(分别为16.9%和5.9%,P<0.05)。有门静脉癌栓组的ERCC1阳性率为66.7%,无癌栓组为42.3%,差异具有统计学意义(P<0.05);但ERCC1阳性表达与性别等其他临床病理特征均无关(P均>0.05)。接受根治术的184例患者中,ERCC1阳性率随着中位复发转移时间(TTR/M)的延长而显著降低(x~2=9.630,P=0.047);ERCCI阳性组TTR/M为10.3个月,而阴性表达组为20.3个月,差异具有统计学意义(P<0.05)。多因素生存分析显示,术前谷草转氨酶>2.5倍正常值、病灶最大径>5 cm和ERCCl阳性表达是HCC患者术后复发转移风险增加的独立危险因素。结论:肝癌组织中ERCCl阳性率显著高于癌旁肝组织及非癌肝组织,其阳性表达与门静脉癌栓形成及术后复发、转移风险增高显著相关。
Objective: To investigate the expression and clinical significance of excision repair cross-complementing gene 1 (ERCC1) in primary hepatocellular carcinoma (HCC) tissue. Methods: The expression of ERCC1 in 226 cases of HCC cancer tissues, 65 cases of HCC adjacent tissues and 17 cases of non-cancerous liver tissues were detected by immunohistochemical method, and the relationship between ERCC1 and clinicopathological factors and prognosis of HCC was analyzed. The positive rate of ERCC1 was 44.2% (100/226), which was significantly higher than that in para-cancer and non-cancer normal liver tissues (16.9% and 5.9%, respectively, P<0.05). The positive rate of ERCC1 in patients with portal vein tumor thrombosis was 66.7% and 42.3% in patients without tumor thrombosis (P<0.05). However, the positive expression of ERCC1 was not associated with any other clinicopathologic features (P>0.05). ). Among the 184 patients who underwent radical resection, the positive rate of ERCC1 decreased significantly with the prolongation of median recurrence and metastasis (TTR/M) (x~2=9.630, P=0.047); TTR/M of ERCCI positive group was 10.3 Months, while the negative expression group was 20.3 months, the difference was statistically significant (P<0.05). Multivariate survival analysis showed that preoperative serum aspartate aminotransferase >2.5 times normal, lesion maximum diameter >5 cm, and positive expression of ERCCl were independent risk factors for increased recurrence and metastasis risk in patients with HCC. Conclusion: The positive rate of ERCCl in HCC tissue was significantly higher than that in para-cancerous liver tissue and non-cancerous liver tissue. The positive expression of ERCCl was significantly related to the formation of portal vein tumor thrombus, and the risk of postoperative recurrence and metastasis.