,Protective Effect of Thrombopoietin on Myocardial Cell in Vitro

来源 :岭南心血管病杂志(英文版) | 被引量 : 0次 | 上传用户:shnoonkids
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
To investigate the protective effect of thrombopoietin (TPO) on myocardial cells in vitro. Methods H9C2 cell line was maintained in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 10% calf serum.Beating cells from heart ventricles of neonatal heart were cultured at an in vitro system. Apoptosis of the cell line above was induced by treatment of doxorubicin (DOX) and was blocked by TPO. Cell survival rate of H9C2 cell was measured by the MTr assay. Changes of beating rate of neonatal myocardial cells were captured by digital camera and beating rate was calculated. Flow cytometry was employed to study anti-apoptotic effect of TPO by staining JC-1 protein to H9C2 cell. Results MTT assay demonstrated that doxorubicin reduced cell survival rate by 73.8% + 1.1%, 50 ng * a dose-dependent manner. Beating rate of primary neonatal myocardial cells also decreased to 15% ± 8% at 48 h, 100 11% ± 6% ( P< 0. 05 ). Conclusions TPO has protective effect on myocardial cells in vitro. Anti-apoptosis is one of the mechanisms by which TPO protects injured heart.
其他文献
Background Coronary slow flow (CSF) during primary percutaneous coronary intervention (PCI) is closely related to the prognosis of patients with acute myocardia
农业面源污染是最为重要且分布最为广泛的面源污染,据三峡库区1990年的统计资料表明,90%的悬浮物来自农田径流,氮、磷大部分来源于农田径流。三峡库区人口密集,巨大的人口压力,使农业土地资源的开发已接近超强度利用,化肥农药的施用成为提高土地产出水平的重要途径,因此,面源污染的控制关系到农业及区域社会经济的可持续发展。 三峡大坝建成后,库区流水区断面流速只有0.04m/s,比天然河道断面平均流速
师承教育在中医药人才培养中占有举足轻重的地位,其正在全面融入中医教育.为了探索适应中医药人才成长规律的教育模式,经过15年的实践,南京中医药大学逐步形成了包括在校理论
作为一名基层通讯员,怎样才能吃透“三头”(即上头的方针政策、下头的典型事例、报纸的宣传动态),写出有价值的新闻稿件呢? 过去,我下乡采访,由于只注意下头一些有局限性的
本文在对籼稻恢复系褐飞虱及白叶枯病抗性鉴定的基础上,对若干恢复系的褐飞虱抗性遗传及机理,对白叶枯病抗性遗传以及农艺性状配合力作了初步研究。主要研究结果如下: 1.通过
该研究采用基因工程的手段将1-磷酸甘露醇脱氢酶(mtlD)基因和甜菜碱醛脱氢酶(BADH)基因转入早稻品种,以期培育出耐盐的转基因旱稻,解决盐碱地的开发利用问题,同时带动旱稻的
Objectives To detection of chlamydia pneumoniae (Cpn) DNA in the circulating mononuclear cell fractions of coronary heart disease and to investigate the associa
Objectives To investigate the effects of testosterone enanthate(TE) on serum lipids and lipoproteins metabolism and the expression of androgen receptor ( AR), e
Objective The current study was designed to investigate the features of a family with familial hypercholesterolemia(FH). Methods Twenty members of three generat
小麦收获前穗发芽不仅影响产量,而且严重影响加工品质、粮食存储及下年的播种质量,造成不同程度的经济损失。近年来,由于阴雨潮湿的年份增加以及穗发芽抗性较弱的白粒品种种植面积的扩大等因素,生产上的穗发芽现象日趋严重。为此,本研究对穗发芽较为严重的西南冬麦区、长江中下游冬麦区以及东北春麦区自50年代以来生产上推广的主要小麦品种345份,利用穗发芽率和面粉降落值两个指标以及等电聚焦电泳技术综合分析了小麦品种