【摘 要】
：
南方水稻黑条矮缩病毒(SRBSDV)和水稻黑条矮缩病毒(RBSDV)在病害症状、传播介体及寄主等方面非常相似,田间很难对其进行诊断及鉴定。根据SRBSDV与RBSDV外壳蛋白(CP)基因核苷
【机 构】
：
江西农业大学农学院/作物生理生态与遗传育种教育部重点实验室,
论文部分内容阅读
南方水稻黑条矮缩病毒(SRBSDV)和水稻黑条矮缩病毒(RBSDV)在病害症状、传播介体及寄主等方面非常相似,田间很难对其进行诊断及鉴定。根据SRBSDV与RBSDV外壳蛋白(CP)基因核苷酸序列差异设计特异性引物,建立一种快速、准确的双重PT-PCR鉴别方法,为SRBSDV和RBSDV的流行监测提供技术支持。
The southern rice black-streaked dwarf virus (SRBSDV) and the rice black streaked dwarf virus (RBSDV) are very similar in disease symptoms, transmission mediators and hosts, and are difficult to diagnose and identify in the field. According to the differences of nucleotide sequences between the coat protein (CP) gene of SRBSDV and RBSDV, we designed a rapid and accurate double PT-PCR method to identify the specific primers and provided technical support for the epidemiological surveillance of SRBSDV and RBSDV.
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