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本文报道以脂质体包被的丝状血凝素(FHA)和脱毒的百日咳毒素(PT)给小鼠口服后诱发的特异性全身和分泌性免疫应答。 纯化的FHA和PT通过溶剂稀释微载体技术包被在多层磷脂囊内,该磷脂囊由卵磷脂、磷脂酰乙醇胺、磷脂酸、中性脂按8:1:0.7:0.3的比例组成,经凝胶过滤去除游离FHA和PT。作者选用5～6周龄雌性BALB/c小鼠分别口服包被FHA的脂质体、包被PT的脂质体、包被FHA和PT的脂质体、游离FHA、游离PT、游离的FHA和PT,以及未包被的脂质体(作为对照)。每次剂量为4μg蛋白,0和4天基础免疫,第30天以同样剂量加强免疫。加强免疫后10天,采集血液和肺灌洗液,应用ELISA测定血清和肺灌洗液中特异性抗FHA和PT抗体滴
This article reports the specific systemic and secretory immune responses elicited after oral administration of liposome-coated filamentous hemagglutinin (FHA) and detoxified pertussis toxin (PT) to mice. Purified FHA and PT were coated in a multilayer phospholipid vesicle by a solvent-dilute microcarrier technique consisting of lecithin, phosphatidylethanolamine, phosphatidic acid, neutral lipid in a ratio of 8: 1: 0.7: 0.3 and Gel filtration removes free FHA and PT. The authors selected 5 to 6-week-old female BALB / c mice were orally coated with liposomes of FHA, liposomes coated with PT, liposomes coated with FHA and PT, free FHA, free PT, free FHA And PT, as well as uncoated liposomes (as control). Each dose of 4μg of protein, 0 and 4 days of basic immunization, the same dose on the 30th day to enhance immunity. Ten days after booster immunization, blood and lung lavage fluid were collected and the specific anti-FHA and PT antibody drops in serum and lung lavage fluid were measured by ELISA