论文部分内容阅读
目的探讨microRNA分子miR-203对食管鳞癌Eca109细胞增殖及侵袭能力的影响。方法根据人源miR-203序列,设计并合成其双链模拟物。通过脂质体转染将miR-203模拟物分子导入食管鳞癌Eca109细胞中,转染无关microRNA模拟物作为对照。测定2组细胞的细胞倍增时间、细胞凋亡率以及侵袭细胞率,观察miR-203对Eca109细胞增殖及侵袭能力的影响。结果 Eca109细胞转染miR-203后其细胞倍增时间为(26.1±0.5)h,较对照组(24.2±0.6)h明显延长(P<0.01);其凋亡细胞比例较对照组增加[(4.5±0.4)%vs(3.7±0.4)%,P<0.05];Transwell小室侵袭实验显示转染miR-203模拟物后,该组的侵袭细胞率明显低于对照组[(39.2±5.8)%vs(49.5±6.8)%,P<0.05]。结论 miR-203能抑制Eca109细胞的增殖和侵袭能力,提示miR-203可能是食管鳞癌生物治疗的潜在靶点。
Objective To investigate the effect of microRNA miR-203 on the proliferation and invasion of esophageal squamous carcinoma cell line Eca109. Methods Based on human miR-203 sequence, double-stranded mimics were designed and synthesized. The miR-203 mimic molecule was transfected into esophageal squamous carcinoma cell line Eca109 by lipofectamine transfection, and transfected with unrelated microRNA mimics as a control. The cell doubling time, apoptosis rate and cell invasion rate of the two groups of cells were measured to observe the effect of miR-203 on the proliferation and invasion ability of Eca109 cells. Results The cell doubling time of Eca109 cells transfected with miR-203 was (26.1 ± 0.5) h, which was significantly longer than that of the control group (24.2 ± 0.6) h (P <0.01) ± (39 ± 5.8)% vs (± 0.4)% vs (3.7 ± 0.4)%, P <0.05]. Transwell chamber invasion assay showed that the invasion rate of miR-203 mimics was significantly lower than that of the control group (49.5 ± 6.8)%, P <0.05]. Conclusion miR-203 can inhibit the proliferation and invasion of Eca109 cells, suggesting miR-203 may be a potential target for biological treatment of esophageal squamous cell carcinoma.