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以那格列奈(Nateglinide)为模板分子,丙烯酰胺为功能单体,采用原位聚合法在功能化多壁碳纳米管(MWNTs)修饰的玻碳电极表面合成了那格列奈分子印迹聚合物颗粒,制成了电流型那格列奈电化学传感器。利用循环伏安法(CV)及差示脉冲法(DPV)对传感器性能进行了研究并对影响传感器响应的条件如底液、p H值等进行了优化。结果表明:制得的传感器对那格列奈的测定具有较高的灵敏性和选择性,在p H=4.0的PBS缓冲液中,那格列奈检测线性范围为2.0~160μg/m L,检测限为1.0μg/m L(S/N=3)。该方法被成功用于血样中那格列奈的测定,只需对样品进行简单处理,加标回收率为81.6%~94.2%。
Nateglinide as a template molecule and acrylamide as functional monomer were synthesized by in-situ polymerization on the surface of glassy carbon electrode modified with functionalized multi-walled carbon nanotubes (MWNTs) Particles, made of current-type Nateglinide electrochemical sensors. The performance of the sensor was studied by cyclic voltammetry (CV) and differential pulse (DPV), and the conditions that affected the response of the sensor, such as bottom solution and p H value, were optimized. The results showed that the prepared sensor has high sensitivity and selectivity for the determination of nateglinide. The linear range of nateglinide in the PBS solution with p H = 4.0 was 2.0 ~ 160μg / m L, The detection limit was 1.0 μg / mL (S / N = 3). The method was successfully applied to the determination of nateglinide in blood samples. The simple sample processing was simple and the recoveries were 81.6% ~ 94.2%.