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目的观察不同剂量的几种化学免疫调节剂免疫小鼠诱导抗弓形虫感染作用。方法不同剂量匹多莫德、沙利度胺、匹多莫德加沙利度胺分别灌胃小鼠,共免疫14次,对照组用PBS灌胃,检测其免疫效果,并观察其受到弓形虫攻击感染后的生存情况。结果匹多莫德、匹多莫德加沙利度胺使小鼠IFN-r、CD4+细胞数量升高(P<0.01),CD4+与CD8+比值增加,各组均未检测到IL-4;沙利度胺使小鼠CD8+细胞数量明显增加(P<0.01),CD4+与CD8+比值减少,各组T细胞增殖活性与PBS对照组比较明显增强(P<0.05),且匹多莫德20mg加沙利度胺1.0mg组T细胞增殖活性最明显。小鼠攻击试验表明,20mg匹多莫德加1.0mg沙利度胺组小鼠存活时间明显长于匹多莫德、沙利度胺以及PBS对照组。结论匹多莫德20mg加沙利度胺1.0mg可诱导更有效的抗弓形虫感染保护作用。
Objective To observe the anti-Toxoplasma infection induced by different doses of several chemical immunomodulatory agents in mice. Methods Pidotimod, thalidomide, and pidotimod plus thalidomide were administered intragastrically to mice for 14 times, respectively. The control group was given gavage with PBS, and the immune effect was tested. Post-infection survival. Results Pidotimod and pidotimod plus thalidomide increased the numbers of IFN-γ and CD4 + cells in mice (P <0.01), and the ratio of CD4 + / CD8 + increased. IL-4 was not detected in each group. (P <0.01), and the ratio of CD4 + to CD8 + decreased. The proliferation activity of T cells in each group was significantly higher than that in PBS control group (P <0.05), and Pidotimod 20 mg plus thalidomide The highest proliferative activity of T cells was found in the group of 1.0 mg amine. The mouse challenge test showed that the survival time of 20 mg pidotimod plus 1.0 mg thalidomide group was significantly longer than that of podolimod, thalidomide and PBS control group. Conclusion Pidotimod 20 mg plus thalidomide 1.0 mg induced more effective protection against Toxoplasma gondii infection.