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Plasminogen activator inhibitor 1 (PAI 1) is one of important coagulant factors. Endotoxin lipopolysaccharide (LPS) induces thrombosis by stimulating PAI 1 secretion of vascular cells (EC). Using sandwich enzyme linked immunosorbent assay (ELISA) and Northern blot, was investigated the effects of Chinese medicine ligustrazini on PAI 1 expression in EC and LPS stimulated EC. The results showed that ligustrazini inhibited both basal and LPS induced PAI 1 mRNA expression in EC. The effect of ligustrazini on LPS induced PAI 1 secretion worked in a dose dependent manner. This study provided theoretic and experimental evidence for use of ligustrazini against septic shock and cardiovascular diseases.
Endogenxin lipopolysaccharide (LPS) induces thrombosis by stimulating PAI 1 secretion of vascular cells (EC). Using sandwich enzyme linked immunosorbent assay (ELISA) and Northern blot, was investigated the Effects of Chinese medicine ligustrazini on PAI 1 expression in EC and LPS stimulated EC. The results showed that ligustrazini inhibited both basal and LPS induced PAI 1 mRNA expression in EC. The effect of ligustrazini on LPS induced PAI 1 process worked in a dose dependent manner. This study provided theoretic and experimental evidence for use of ligustrazini against septic shock and cardiovascular diseases.