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为阐明放射损伤时骨髓造血基质细胞支持粒 -巨噬系祖细胞造血能力的变化。采用小鼠骨髓基质细胞体外融合培养和粒 -巨噬系祖细胞集落 (CFU -GM )培养法 ,观察 5Gy照射对基质细胞支持小鼠CFU -GM生长的影响。结果表明 ,经 5Gy照射后 ,骨髓基质细胞支持正常粒 -巨噬系祖细胞造血的能力明显下降 ,以伤后 3- 5d最为显著 ,伤后 10d仍未恢复至正常 ;当骨髓基质细胞和粒 -巨噬系祖细胞均受到致伤因素作用时 ,照射组的CFU -GM形成能力下降更为明显 ,至正常对照组的 10 %以下。说明放射损伤后骨髓基质细胞的功能受到了不同程度的损伤 ,因此 ,在治疗由此所引起造血功能障碍的同时 ,应加强促进基质细胞修复的措施 ,可能会取得更满意的疗效。
To clarify the hematopoietic capacity of bone marrow hematopoietic stromal cells supporting granule-macrophage progenitor cells during radiation injury. Mouse bone marrow stromal cells in vitro fusion culture and granulocyte macrophage-derived progenitor colony (CFU-GM) culture method was used to observe the effects of 5Gy irradiation on stromal cell-supported CFU-GMM. The results showed that the ability of bone marrow stromal cells to support hematopoiesis of normal granulocyte macrophage-derived progenitor cells was significantly decreased after 5 Gy irradiation, most notably 3-5 days after injury, but not returned to normal 10 days after injury. When bone marrow stromal cells and granulocytes - Macrophage-derived progenitor cells were injured by factors, the irradiation group CFU-GM formation decreased more significantly, to 10% of the normal control group. It shows that the function of bone marrow stromal cells is damaged to varying degrees after radiation injury. Therefore, we should strengthen the measures of promoting the repair of hematopoietic cells while treating the hematopoietic dysfunction caused by them, which may lead to more satisfactory curative effect.