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动脉成形术后血管弹性回缩及平滑肌细胞增殖是血管再狭窄的重要原因。本文着重探讨表皮生长因子（ＥＧＦ）对大鼠冠状动脉收缩作用及其对血管平滑肌细胞（ＳＭＣ）分裂增殖的影响，并观察细胞表面ＥＧＦ受体的调节特点。应用不同浓度的ＥＧＦ作用于内膜损伤后的冠状动脉环，观察收缩强度的变化，分析不同浓度的ＥＧＦ对培养的ＳＭＣ３Ｈ－ＴｄＲ掺入率及分裂增殖的影响，并利用１２５Ｉ－ＥＧＦ分析受体密度变化。结果显示，浓度为１×１０－１０－１×１０－６ｍｏｌ／Ｌ的ＥＧＦ可引起大鼠冠状动脉呈剂量依赖性收缩反应（ｒ＝０．９１６，Ｐ＜０．００１），其Ｅｍａｘ为１１０．５ｍｇ，ＥＣ５０为９．６７ｎｍｏ／Ｌ。在同时存在０．５％的胎牛血清（ＦＣＳ）时，ＥＧＰ能促进ＳＭＣ的３Ｈ－ＴｄＲ掺入率，该作用与剂量呈正相关（ｒ＝０．８５２，Ｐ＜０．０５）。ＥＧＦ在０．５％ＦＣＳ中有显著的促ＳＭＣ分裂增殖作用，与１０％ＦＣＳ对照有显著差异（Ｐ＜０．００１）。１×１０－１３ｍｏｌ／ＬＥＧＦ单独存在时，未显示出明显致分裂活性。在显著分裂增殖的ＳＭＣ中，每个细胞表面受体约是缓慢增殖细胞的１１倍（４２００／３８００），ＥＧＦ受体明显上调，故ＥＧＦ对增殖期细胞作用尤为突出?
Vascular elastic retraction and smooth muscle cell proliferation after angioplasty are important causes of restenosis. This article focuses on the effect of epidermal growth factor (EGF) on the contraction of rat coronary arteries and its effect on the proliferation and proliferation of vascular smooth muscle cells (SMCs), and observes the regulatory characteristics of EGF receptors on the cell surface. The effect of different concentrations of EGF on the SMC3H-TdR incorporation rate and the proliferation and proliferation of cultured SMC3H-TdR were observed by using different concentrations of EGF on the coronary artery rings after intimal injury, and the changes of the contraction intensity were observed. Density changes. The results showed that the concentration of 1 × 10-10-1 × 10-6mol / L of EGF can cause a dose-dependent coronary artery contraction (r = 0.916, P <0.001), the Emax of 110 .5mg, EC50 9.67nmo / L. In the presence of 0.5% fetal bovine serum (FCS), EGP promoted 3H-TdR incorporation in SMCs with a positive correlation with dose (r = 0.852, P <0.05). EGF significantly promoted SMC cleavage in 0.5% FCS, which was significantly different from that of 10% FCS control (P <0.001). In the presence of 1 × 10-13 mol / L EGF alone, no significant mitogenic activity was shown. In SMCs with significantly proliferating cells, the surface receptor per cell is about 11 times higher than that of slowly proliferating cells (4200/3800), and the EGF receptor is significantly upregulated. Therefore, the effect of EGF on proliferating cells is particularly prominent.