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MoS2纳米片能够有效地催化过氧化氢氧化底物和3,3’,5,5’-四甲基联苯胺(TMB),产生颜色变化,利用亚硫酸根能够消耗过氧化氢而降低反应体系吸光度的原理,建立一种亚硫酸根的快速比色传感体系并用于葡萄酒样品的检测。实验考察并优化了反应时间、H2O2浓度、pH值和温度等影响参数,在最优条件下(反应时间30min、H2O2 浓度100μmol?L-1、pH值7.0、温度25 oC),亚硫酸根浓度在5.0 ~120.0 μmol?L-1范围内与吸光度具有良好的线性关系,检测限(3σ/k)为0.5 μmol?L-1。采用所建立的方法测定葡萄酒中的亚硫酸根,样品加标回收率为96.6%~106.5%。
MoS2 nanosheets can effectively catalyze the oxidation of hydrogen peroxide with 3,3 ’, 5,5’-tetramethylbenzidine (TMB) to produce a color change, and the use of sulfite can consume hydrogen peroxide and reduce the reaction system Absorbance principle, the establishment of a sulfite fast colorimetric sensing system and used for the detection of wine samples. Under the optimal conditions (reaction time 30min, H2O2 concentration 100μmol? L-1, pH value 7.0, temperature 25oC), the concentration of sulfite In the range of 5.0 ~ 120.0 μmol? L-1, there was a good linear relationship between absorbance and absorbance. The detection limit (3σ / k) was 0.5 μmol? L-1. The proposed method was applied to the determination of sulfite in wine. The spiked recoveries were 96.6% -106.5%.