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[目的]对从内蒙古自治区呼伦贝尔盟海拉尔市苜蓿根际土壤中分离出的小杆线虫(Rhabditis(Oscheius)sp.)的生物学特性进行测定。[方法]以大蜡螟(Galleria mellonella)和黄粉甲(Tenebrio molitor)老熟幼虫为寄主,测定小杆线虫的侵染力;以水浴方法测定小杆线虫的耐热能力。[结果]小杆线虫在10、20和40 IJ/s虫的剂量下侵染力较低;但在80、160、320和640 IJs/虫的剂量下侵染力较高,侵染大蜡螟96 h、侵染黄粉甲120 h的校正死亡率均达90%以上;在80 IJs/虫的剂量下侵染力最高,侵染120 h,寄主的校正死亡率达100%。在所测试的较高剂量下,小杆线虫对黄粉虫的侵染效果低于对大蜡螟的侵染效果。侵染大蜡螟达到90%上校正死亡率的侵染效果需96 h,而侵染黄粉虫达到同样的效果则需120 h。浓度1 000～5 000 IJ/ml的小杆线虫在36℃条件下水浴2 d,有25%左右的个体存活,水浴6 d,仍有3%～9%的个体存活,至第9天,线虫个体全部死亡;在38℃下水浴6h、40℃下水浴2 h,线虫个体全部死亡。[结论]小杆线虫具有较强的侵染力和耐热能力,具有开发应用潜能。
[Objective] The biological characteristics of Rhabditis (Oscheius sp.) Isolated from rhizosphere soil of alfalfa in Hailaer, Inner Mongolia Autonomous Region were determined. [Method] With the larvae of Galleria mellonella and Tenebrio molitor as host, the infectivity of C. elegans was determined. The heat resistance of C. elegans was determined by water bath method. [Result] The larval nematodes had lower infectivity at doses of 10, 20 and 40 IJ / s, but higher infectivity at doses of 80, 160, 320 and 640 IJs / The corrected mortalities reached 96% at 120 h for 96 h and reached the peak at 120 h. The highest infectivity was observed at 80 IJs / worm and the corrected host mortality was 100% at 120 h. In the higher doses tested, the infection by C. elegans to Tenebrio molitor was less effective than the A. gigasia. It takes 96 h to reach the 90% corrected mortality rate for A. lanceolata and 120 h for the same effect on T. molitor. At the concentration of 1 000-5 000 IJ / ml, the juvenile C. elegans had a water bath for 2 days at 36 ℃, and about 25% of the individuals survived. After 6 days of water bathing, 3% -9% of the individuals remained alive. By the 9th day, Nematodes all died; water bath at 38 ℃ 6h, water bath at 40 ℃ 2 h, all nematode individuals died. [Conclusion] The juvenile nematode has strong infectivity and heat tolerance, and has potential for development and application.