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目的:建立HPLC法测定固经丸中芍药苷的含量。方法:ZORBAX SB-C_(18)分析柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.05 mol·L~(-1)磷酸二氢钾溶液-三乙胺(28:72:0.5);检测波长:230 nm;流速:1.0 ml·min~(-1),柱温:40℃。结果:芍药苷在0.44～3.96μg范围内的线性关系良好(r=0.9999),平均加样回收率为99.2%(n=5),RSD 1.2%(n=5)。结论:该方法准确,灵敏度高,重现性好。
Objective: To establish HPLC method for determination of paeoniflorin in Gujing Pill. Methods: ZORBAX SB-C_(18) analytical column (250 mm×4.6 mm, 5 μm); mobile phase: methanol-0.05 mol·L -1 potassium dihydrogen phosphate solution - triethylamine (28:72:0.5) Detection wavelength: 230 nm; flow rate: 1.0 ml·min -1; column temperature: 40°C. Results: The calibration curve showed good linearity in the range of 0.44 to 3.96 μg (r=0.9999). The average recovery was 99.2% (n=5) and RSD 1.2% (n=5). Conclusion: This method is accurate, high sensitivity and good reproducibility.