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目的评价无精子症患者睾丸穿刺中检测支持细胞指数的意义。方法采用Johnson评分法评价睾丸活检组织生精情况;采用瑞-姬染色,人工判读睾丸穿刺液涂片中各期生精细胞和支持细胞,并对其进行分类计数。结果睾丸活检组织随着Johnson评分降低,支持细胞形态结构越不规则;睾丸穿刺中生精正常组9例,支持细胞指数范围均在20%～40%之间;生精障碍组15例,其中11例支持细胞指数范围在80%～150%,4例支持细胞指数范围在30%以下;生精阻滞组9例,支持细胞指数范围均在150%～500%之间,1例为15%。结论生精正常组与生精障碍组、生精阻滞组的支持细胞指数差异明显,无精子症患者睾丸穿刺报告中支持细胞指数具有重要意义。
Objective To evaluate the significance of detecting the index of supporting cells in testicular puncture in patients with azoospermia. Methods Johnson ’s score was used to evaluate the spermatogenesis of testis biopsies. The spermatogenic cells and supporting cells of testis puncture fluid were manually digested by Rui - Ji staining, and were classified and counted. Results In the testis biopsy, the morphology of the supporting cells was more irregular with the decrease of the Johnson score. In the normal group, there were 9 cases of normal spermatogenesis in the testis and the range of the index of the supporting cells was between 20% and 40% The index of supportive cells was in the range of 80% -150% in 11 cases, the index of supportive cells in 4 cases was less than 30%, the group of spermatogenic arrest was in 9 cases, the index of supporting cells was in the range of 150% -500% %. Conclusion The difference of the index of supporting cells between the normal group and the group of spermatogenic dyspnea and spermatogenic group is significant, and the index of supporting cells in the testicular puncture report of azoospermia is of great significance.