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目的探讨双酚A对大鼠背根神经节(DRG)神经元钙离子通道影响。方法 SD大鼠,周龄4～6周,采用酶消化法急性分离大鼠背根神经节神经元,分别采用全细胞膜片钳技术和激光共聚焦技术记录钙电流和KCl激发钙瞬变的变化。结果双酚A(0.1、1、10、100μmol/L)呈浓度依赖性抑制大鼠背根神经节神经元钙电流,对钙通道的半数最大抑制浓度(IC50)为11.41μmol/L;10μmol/L双酚A显著改变Ca2+通道的激活特性,电流激活曲线去极化方向移动(P<0.05),该浓度双酚A同样可以抑制50 mmol/L KCL激发瞬时胞内钙浓度增加。结论双酚A对大鼠背根神经节神经元钙离子通道具有抑制作用。
Objective To investigate the effect of bisphenol A on calcium channel in rat DRG neurons. Methods Sprague-Dawley rat ganglion neurons were isolated from rat SD rats at 4 weeks to 6 weeks of age. The changes of calcium current and KCl-induced calcium transients were recorded by whole-cell patch clamp and confocal laser scanning confocal microscope. . Results Bisphenol A (0.1, 1, 10, 100 μmol / L) inhibited the calcium currents in dorsal root ganglion neurons in a concentration-dependent manner with the IC50 of 11.41 μmol / L and 10 μmol / L bisphenol A significantly changed the activation characteristics of Ca2 + channel, the current activation curve depolarization direction of movement (P <0.05), the concentration of bisphenol A can also inhibit 50 mmol / L KCL-induced transient intracellular calcium concentration increased. Conclusion Bisphenol A can inhibit the calcium channel of dorsal root ganglion neurons in rats.