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为探讨去整合素-金属蛋白酶17(disintegrin and metalloproteinase 17,ADAM17)对人胶质瘤细胞增殖和迁移的影响及其调控机制,该研究用ADAM17 sh RNA质粒转染ADAM17高表达的胶质瘤细胞U87MG、U251MG,用过表达ADAM17载体转染ADAM17低表达细胞SW1783,q-PCR和Western blot检测ADAM17表达水平的变化,同时检测ADAM17和Hippo信号通路中相关蛋白质表达水平,CCK-8法分析细胞增殖能力并绘制生长曲线,划痕实验检测细胞迁移率。结果表明,下调ADAM17后,U87MG、U251MG细胞增殖和迁移能力减弱,而Hippo信号通路中MST2、p-MOB1、p-YAP蛋白表达水平升高;上调ADAM17后,SW1783细胞增殖和迁移能力增强,而MST2、p-MOB1、p-YAP蛋白表达水平降低。结果说明,ADAM17在脑胶质瘤细胞的增殖和迁移中发挥了重要作用,这可能是通过抑制Hippo信号通路来实现的,MST2被抑制,p-MOB1水平降低,从而YAP的磷酸化水平也降低,促进了胶质瘤细胞的增殖和迁移。
To investigate the effect and mechanism of disintegrin and metalloproteinase 17 (ADAM17) on the proliferation and migration of human glioma cells, ADAM17 shRNA plasmid was used to transfect ADAM17-overexpressing glioma cells U87MG and U251MG. ADAM17 overexpression ADAM17 vector was transfected into SW1783 cells. The expression of ADAM17 was detected by q-PCR and Western blot. The protein expression of ADAM17 and Hippo was detected by qPCR and Western blot. CCK-8 was used to analyze cell proliferation Ability and draw growth curve, scratch test to detect cell migration. The results showed that the expression of MST2, p-MOB1 and p-YAP in U87MG and U251MG cells was decreased after ADAM17 was down-regulated, while the expression of MST2, p-MOB1 and p-YAP in Hippo signaling pathway was up-regulated. MST2, p-MOB1, p-YAP protein expression decreased. The results showed that ADAM17 plays an important role in the proliferation and migration of glioma cells, which may be through the inhibition of Hippo signaling pathway, MST2 is inhibited, the level of p-MOB1 is reduced, and the phosphorylation of YAP is also reduced , Promote glioma cell proliferation and migration.